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  • 1
    Electronic Resource
    Electronic Resource
    Influence of Axial Coordination on the g-Factor Anisotropy of the Tetraimine Macrocyclic Cobalt(II) Complex [Co(C10H20N8)Cl(H2O)]Cl.cntdot.H2O (1994)
    s.l. : American Chemical Society
    Inorganic chemistry 33 (1994), S. 3188-3190 
    Details
    ISSN: 1520-510X
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ic00092a029
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of temperature and process technology on the occurrence of Aeromonas species and hygienic indicator organisms in drinking water production plants (1995)
    Springer
    Microbial ecology 30 (1995), S. 203-218 
    Details
    ISSN: 1432-184X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The occurrence of Aeromonas spp. and hygienic indicator organisms in raw and treated waters of five drinking water production plants in Flanders (Belgium) was surveyed over a period of 17 months. Aeromonads were isolated on ampicillin-dextrin agar (ADA) and further identified by gas-liquid chromatographic analysis of their cellular fatty acid methyl ester (FAME) content. ADA medium was found to be highly specific for the enumeration of Aeromonas spp. In general, Aeromonas counts were very low in untreated groundwater but numbered 104–106 colony-forming units per liter in open storage reservoirs for surface water. Aeromonas spp. were seasonally distributed with maximal densities occurring during the summer. The ecology of Aeromonas in the different waters was studied in relation to the physical, chemical, and microbiological water characteristics. Strongly positive correlations were observed between Aeromonas densities and heterotrophic plate counts, whereas a clearly negative relationship was found with dissolved oxygen. On average, 99.7% of the aeromonads were removed by flocculation-decantation followed by breakpoint chlorination, whereas 98.9% were removed by slow sand filtration. Flocculation-decantation without breakpoint chlorination did not reduce the microbial numbers. At three of four drinking water production plants tested, rapid sand filtration decreased the number of aeromonads and hygienic indicator organisms. At one plant, however, the numbers of Aeromonas and hygienic indicator organisms were high in the sand filter effluents. Increased numbers of aeromonads were also counted in the effluent of the activated carbon filters. Hence, inactivation of Aeromonas spp. by the current process technology appears not sufficient to exclude postchlorination. The survival of aeromonads in certain filter systems may be due to the growth of these bacteria on biodegradable organic material, provided by the decomposition from bacteria, algae, or other sources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00172575
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  • 3
    Electronic Resource
    Electronic Resource
    Metabolic pathways and energetics of the acetone-oxidizing, sulfate-reducing bacterium, Desulfobacterium cetonicum (1995)
    Springer
    Archives of microbiology 163 (1995), S. 188-194 
    Details
    ISSN: 1432-072X
    Keywords: Key words Anaerobic degradation ; Acetone ; Carboxylation ; Energetics ; Sulfate-reducing bacterium ; Desulfobacterium cetonicum ; Citric acid cycle ; Glyoxylate cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Acetone degradation by cell suspensions of Desulfobacterium cetonicum was CO2-dependent, indicating initiation by a carboxylation reaction. Degradation of butyrate was not CO2-dependent, and acetate accumulated at a ratio of 1 mol acetate per mol butyrate degraded. In cultures grown on acetone, no CoA transfer apparently occurred, and no acetate accumulated in the medium. No CoA-ligase activities were detected in cell-free crude extracts. This suggested that the carboxylation of acetone to acetoacetate, and its activation to acetoacetyl-CoA may occur without the formation of free acetoacetate. Acetoacetyl-CoA was thiolytically cleaved to two acetyl-CoA, which were oxidized to CO2 via the acetyl-CoA/carbon monoxide dehydrogenase pathway. The measured intracellular acyl-CoA ester concentrations allowed the calculation of the free energy changes involved in the conversion of acetone to acetyl-CoA. At in vivo concentrations of reactants and products, the initial steps (carboxylation and activation) must be energy-driven, either by direct coupling to ATP, or coupling to transmembrane gradients. The ΔG' of acetone conversion to two acetyl-CoA at the expense of the energetic equivalent of one ATP was calculated to lie very close to 0 kJ (mol acetone)–1. Assimilatory metabolism was by an incomplete citric acid cycle, lacking an activity oxidatively decarboxylating 2-oxoglutarate. The low specific activities of this cycle suggested its probable function in anabolic metabolism. Succinate and glyoxylate were formed from isocitrate by isocitrate lyase. Glyoxylate thus formed was condensed with acetyl-CoA to form malate, functioning as an anaplerotic sequence. A glyoxylate cycle thus operates in this strictly anaerobic bacterium. Phosphoenolpyruvate (PEP) carboxykinase formed PEP from oxaloacetate. No pyruvate kinase activity was detected. PEP presumably served as a precursor for polyglucose formation and other biosyntheses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305352
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  • 4
    Electronic Resource
    Electronic Resource
    Disproportionation of inorganic sulfur compounds by the sulfate-reducing bacterium Desulfocapsa thiozymogenes gen. nov., sp. nov. (1996)
    Springer
    Archives of microbiology 166 (1996), S. 184-192 
    Details
    ISSN: 1432-072X
    Keywords: Key words Sulfur ; Thiosulfate ; Sulfite ; Disproportionation (thiosulfate ; sulfite) ; Sulfate ; reduction ; Ferric iron ; Desulfocapsa thiozymogenes ; Desulfobulbus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new strictly anaerobic, gram-negative bacterium was isolated from the sediment of a freshwater lake after enrichment with thiosulfate as the energy source. The strain, named Bra2 (DSM 7269), is able to grow by disproportionation of thiosulfate or sulfite to sulfate plus sulfide. Elemental sulfur is also disproportionated to sulfate and sulfide, but this only supports growth if free sulfide is chemically removed from the culture, e.g., by precipitation with amorphous ferric hydroxide. Growth is also possible by coupling the reduction of sulfate to sulfide with the oxidation of ethanol, propanol, or butanol to the corresponding fatty acid. The cells are rod-shaped, motile, and have genomic DNA with a mol% G+C content of 50.7. Cytochromes are present, but desulfoviridin is not. The new strain was shown to be related to, but distinct from members of the genus Desulfobulbus on the basis of physiological characteristics and by comparative sequence analysis of its 16S rDNA. Strain Bra2 is described as the type strain of a new taxon, Desulfocapsa thiozymogenes gen. nov., sp. nov.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050374
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  • 5
    Electronic Resource
    Electronic Resource
    Clostridium viride sp. nov., a strictly anaerobic bacterium using 5-aminovalerate as growth substrate, previously assigned to Clostridium aminovalericum (1994)
    Springer
    Archives of microbiology 162 (1994), S. 387-394 
    Details
    ISSN: 1432-072X
    Keywords: Key words     Clostridium viride sp. nov. ; Clostridium aminovalericum ; 5-Aminovalerate ; Sulphur reduction ; Anaerobic degradation ; 2 ; 4-Pentadienoyl-CoA reductase ; 5-Hydroxyvaleryl-CoA dehydratase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract      Strain T2–7, a 5-aminovalerate-fermenting bacterium previously classified as Clostridium aminovalericum, was further characterized, both physiologically and phylogenetically. Comparative sequencing analysis of the almost complete 16S rDNA revealed that strain T2–7 forms a distinct lineage within a phylogenetically coherent cluster of gram-positive bacteria currently assigned to the genus Clostridium. Strain T2–7 grew with 5-aminovalerate, 5-hydroxyvalerate, 4-hydroxybutyrate, vinylacetate, and crotonate, and required yeast extract and l-cysteine for growth. Other substrates were not utilized. The fermentation products, depending on the growth substrate, were ammonia, acetate, propionate, butyrate, and valerate. Sulphur was reduced by a mechanism not linked to energy conservation. Other acceptors were not utilized. Cells were gram-positive pointed-ended ovals, motile by means of two subpolar flagella, and possessed a gram-positive cell wall structure with an S-layer of hexagonally arranged subunits of 18.5 nm diameter. The DNA mol% G+C was 41.5. Strain T2–7 (DSM 6836) is proposed as the type strain of a new species, Clostridium viride sp. nov.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050155
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  • 6
    Electronic Resource
    Electronic Resource
    Succinate decarboxylation by Propionigenium maris sp. nov., a new anaerobic bacterium from an estuarine sediment (1995)
    Springer
    Archives of microbiology 164 (1995), S. 29-35 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsPropionigenium maris sp. nov. ; Anaerobic degradation ; Succinate ; Propionate ; Decarboxylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Enrichments on succinate plus yeast extract under anoxic conditions from intertidal mud-flat sediments yielded cultures dominated by oval to round-ended rod-shaped cells. Strain 10succ1, obtained in pure culture, was characterized in detail. The non-motile cells possessed a gram-negative cell wall and did not form spores. Carbohydrates were fermented to formate, acetate, ethanol, and lactate. Succinate was decarboxylated to propionate. Other organic and amino acids were variously fermented to formate, acetate, propionate, and butyrate. Sulfur, sulfate, thiosulfate, and nitrate were not used as electron acceptors. Growth required the presence of yeast extract and at least 5 g/l NaCl, and was possible only in the absence of oxygen. No cytochromes were detected. The DNA base ratio was 40 mol% G+C. Phylogenetically, strain 10succ1 is closely related to Propionigenium modestum, as revealed by 16S rDNA analysis, but is physiologically distinct. Accordingly, strain 10succ1 (DSM 9537) is described as the type strain of a new species of the genus Propionigenium, P. maris sp. nov.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050232
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  • 7
    Electronic Resource
    Electronic Resource
    Force generation and shift of mass between myosin and actin in skinned striated muscle fibres at low calcium concentrations (1998)
    Springer
    European biophysics journal 27 (1998), S. 575-581 
    Details
    ISSN: 1432-1017
    Keywords: Key words Actin ; Myosin ; Muscle fibre ; X-ray diffraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Physics
    Notes: Abstract Skinned muscle fibres from the gracilis muscle of the rabbit were used to record small angle X-ray diffraction spectra under various contractile conditions. The intracellular calcium concentration, expressed as pCa, was varied between 8.0 and 5.74. Equatorial diffraction spectra were fitted by a function consisting of five Gaussian curves and a hyperbola to separate the (1.0), (1.1), (2.0), (2.1) and Z-line diffraction peaks. The hyperbola was used to correct for residual scattering in the preparation. The ratio between the intensities of the (1.1) and (1.0) peaks was defined as the relative transfer of mass between myosin and actin, due to crossbridge formation after activation by calcium. The relation between the ratio and the relative force of the fibre (normalized to the force at pCa 5.74 and sarcomere length 2.0 μm) was linear. At high pCa (from pCa 6.34 to 8.0) no active force was observed, while the ratio still decreased. Sarcomere length was recorded by laser diffraction. The laser diffraction patterns did not show changes in sarcomere length due to activation in the high pCa range (between 8.0 and 6.34). From these results the conclusion is drawn that crossbridge movement occurs even at subthreshold calcium concentrations in the cell, when no active force is exerted. Since no force is generated this movement may be related to crossbridges in the weakly bound state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002490050168
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  • 8
    Electronic Resource
    Electronic Resource
    Haloperidide ( R 3201), a Highly Potent and Selective Anti-emetic Agent in Dogs (1961)
    [s.l.] : Nature Publishing Group
    Nature 190 (1961), S. 911-912 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Both haloperidol and perphenazine are potent inhibitors of the emetic effect of apomorphine in dogs2"3'5. After subcutaneous injection, haloperidol has a quicker onset and a considerably longer duration F--C-CH-CH O Haloperidide (R 3201): R = -C-N; R' = w-Cl Haloperidol (R 1625): R = OH; R' = ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/190911a0
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  • 9
    Electronic Resource
    Electronic Resource
    The molecular weight of the 14S calf lens messenger RNA
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 59 (1974), S. 1157-1164 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(74)80100-2
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  • 10
    Electronic Resource
    Electronic Resource
    Hall constants and magnetoresistance in aluminium single crystals, studied by means of helicon resonances
    Amsterdam : Elsevier
    Journal of Physics and Chemistry of Solids 36 (1975), S. 1261-1265 
    Details
    ISSN: 0022-3697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-3697(75)90201-2
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