ISSN:
1432-072X
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A lipopolysaccharide was isolated from cell walls of Anacystis nidulans by extraction with 45% aqueous phenol at 65°, and further purified by repeated high speed centrifugation. It contains 30–40% of lipid and about 60% of carbohydrate components. The carbohydrate moiety contains predominantly mannose and smaller amounts of galactose, glucose, fucose, rhamnose, 2-keto-3-deoxyoctonate, glucosamine and a second aminosugar. The latter was identified as a 2-amino-2-deoxyheptose with the gluco-configuration from C3 to C7. Thelipid moiety contains glucosamine and fatty acids (C22:0, C18:2, C16:0, C12:0 and C14:βOH). The lipopolysaccharide has a very low phosphate content and does not contain heptose. It shows low pyrogenicity in rabbits and it is not toxic in mice.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00412238
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