Publication Date:
1984-10-12
Description:
A novel eukaryotic hybrid gene has been constructed from the 5' sequence of a rat gene and the bacterial neomycin-resistance gene. After transfection into hamster fibroblasts, the neo transcripts can be induced to high levels by the absence of glucose. Furthermore, this hybrid gene can be regulated by temperature when it is introduced into a temperature-sensitive mutant cell line.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Attenello, J W -- Lee, A S -- CA-27607/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1984 Oct 12;226(4671):187-90.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6484570" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cell Line
;
Cricetinae
;
DNA, Recombinant
;
Drug Resistance, Microbial
;
Fibroblasts
;
*Gene Expression Regulation
;
Genes, Bacterial
;
*Genes, Regulator
;
Glucose/*pharmacology
;
*HSP70 Heat-Shock Proteins
;
Membrane Proteins/biosynthesis/*genetics
;
Mutation
;
Neomycin/pharmacology
;
Rats
;
Temperature
;
Transcription, Genetic
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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