Publication Date:
2004-07-03
Description:
Analysis of developmental plasticity of bone marrow-derived cells (BMDCs) is complicated by the possibility of cell-cell fusion. Here we demonstrate that epithelial cells can develop from BMDCs without cell-cell fusion. We use the Cre/lox system together with beta-galactosidase and enhanced green fluorescent protein expression in transgenic mice to identify epithelial cells in the lung, liver, and skin that develop from BMDCs without cell fusion.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Harris, Robert G -- Herzog, Erica L -- Bruscia, Emanuela M -- Grove, Joanna E -- Van Arnam, John S -- Krause, Diane S -- DK61846/DK/NIDDK NIH HHS/ -- HL073742/HL/NHLBI NIH HHS/ -- HL63357/HL/NHLBI NIH HHS/ -- T32-HL07778/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 2004 Jul 2;305(5680):90-3.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15232107" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bone Marrow Cells/*physiology
;
*Bone Marrow Transplantation
;
Cell Differentiation
;
*Cell Fusion
;
Cobra Cardiotoxin Proteins/pharmacology
;
Elapid Venoms/pharmacology
;
Epithelial Cells/*cytology/metabolism
;
Female
;
Green Fluorescent Proteins
;
Hepatocytes/cytology/metabolism
;
Keratinocytes/cytology/metabolism
;
Keratins/analysis
;
Luminescent Proteins/metabolism
;
Male
;
Mice
;
Mice, Transgenic
;
Muscle Cells/cytology
;
Radiation, Ionizing
;
Recombinases/metabolism
;
Recombination, Genetic
;
Reverse Transcriptase Polymerase Chain Reaction
;
Stem Cells/cytology/*physiology
;
X Chromosome
;
Y Chromosome
;
beta-Galactosidase/biosynthesis/genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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