Publication Date:
1979-03-09
Description:
The restriction enzymes Hpa II and Msp I both recognize the sequence 5'-CCGG (C, cytosine; G, guanine). However, Hpa II cuts mouse liver DNA to fragments four times larger than does Msp I. The size of DNA cut by Msp I is close to that predicted from base composition and nearest neighbor analysis. The most probable explanation of these results is that in mouse the site 5'-CCGG is highly methylated.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Singer, J -- Roberts-Ems, J -- Riggs, A D -- New York, N.Y. -- Science. 1979 Mar 9;203(4384):1019-21.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/424726" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
DNA/*metabolism
;
DNA (Cytosine-5-)-Methyltransferase/metabolism
;
DNA Restriction Enzymes/metabolism
;
Liver/metabolism
;
Methylation
;
Mice
;
Molecular Weight
;
Substrate Specificity
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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