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  • 1
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    Ubiquitin-like small archaeal modifier proteins (SAMPs) in Haloferax volcanii (2010)
    Nature Publishing Group (NPG)
    Details
    Publication Date: 2010-01-08
    Description: Archaea, one of three major evolutionary lineages of life, encode proteasomes highly related to those of eukaryotes. In contrast, archaeal ubiquitin-like proteins are less conserved and not known to function in protein conjugation. This has complicated our understanding of the origins of ubiquitination and its connection to proteasomes. Here we report two small archaeal modifier proteins, SAMP1 and SAMP2, with a beta-grasp fold and carboxy-terminal diglycine motif similar to ubiquitin, that form protein conjugates in the archaeon Haloferax volcanii. The levels of SAMP-conjugates were altered by nitrogen-limitation and proteasomal gene knockout and spanned various functions including components of the Urm1 pathway. LC-MS/MS-based collision-induced dissociation demonstrated isopeptide bonds between the C-terminal glycine of SAMP2 and the epsilon-amino group of lysines from a number of protein targets and Lys 58 of SAMP2 itself, revealing poly-SAMP chains. The widespread distribution and diversity of pathways modified by SAMPylation suggest that this type of protein conjugation is central to the archaeal lineage.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872088/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉   〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2872088/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Humbard, Matthew A -- Miranda, Hugo V -- Lim, Jae-Min -- Krause, David J -- Pritz, Jonathan R -- Zhou, Guangyin -- Chen, Sixue -- Wells, Lance -- Maupin-Furlow, Julie A -- 1S10 RR025418-01/RR/NCRR NIH HHS/ -- P41 RR018502/RR/NCRR NIH HHS/ -- P41 RR018502-07/RR/NCRR NIH HHS/ -- R01 GM057498/GM/NIGMS NIH HHS/ -- England -- Nature. 2010 Jan 7;463(7277):54-60. doi: 10.1038/nature08659.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20054389" target="_blank"〉PubMed〈/a〉
    Keywords: Amino Acid Sequence ; Archaeal Proteins/chemistry/*metabolism ; Gene Deletion ; Glycylglycine/metabolism ; Haloferax volcanii/genetics/metabolism ; Immunoprecipitation ; Mass Spectrometry ; Molecular Sequence Data ; Nitrogen/metabolism ; Proteasome Endopeptidase Complex/genetics/metabolism ; Sequence Alignment ; Sulfur/metabolism ; Ubiquitination ; Ubiquitins/chemistry/*metabolism
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Published by Nature Publishing Group (NPG)
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  • 2
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    E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea (2011)
    National Academy of Sciences
    Details
    Publication Date: 2011-02-28
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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  • 3
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    E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea [Microbiology] (2011)
    National Academy of Sciences
    Details
    Publication Date: 2011-03-16
    Description: Based on our recent work with Haloferax volcanii, ubiquitin-like (Ubl) proteins (SAMP1 and SAMP2) are known to be covalently attached to proteins in archaea. Here, we investigated the enzymes required for the formation of these Ubl-protein conjugates (SAMPylation) and whether this system is linked to sulfur transfer. Markerless in-frame deletions were generated in H. volcanii target genes. The mutants were examined for: (i) the formation of Ubl protein conjugates, (ii) growth under various conditions, including those requiring the synthesis of the sulfur-containing molybdenum cofactor (MoCo), and (iii) the thiolation of tRNA. With this approach we found that UbaA of the E1/MoeB/ThiF superfamily was required for the formation of both SAMP1- and SAMP2-protein conjugates. In addition, UbaA, SAMP1, and MoaE (a homolog of the large subunit of molybdopterin synthase) were essential for MoCo-dependent dimethyl sulfoxide reductase activity, suggesting that these proteins function in MoCo-biosynthesis. UbaA and SAMP2 were also crucial for optimal growth at high temperature and the thiolation of tRNA. Based on these results, we propose a working model for archaea in which the E1-like UbaA can activate multiple Ubl SAMPs for protein conjugation as well as for sulfur transfer. In sulfur transfer, SAMP1 and SAMP2 appear specific for MoCo biosynthesis and the thiolation of tRNA, respectively. Overall, this study provides a fundamental insight into the diverse cellular functions of the Ubl system.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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