Publication Date:
2011-11-05
Description:
The RNA polymerase II (RNAP II) largest subunit contains a C-terminal domain (CTD) with up to 52 Tyr(1)-Ser(2)-Pro(3)-Thr(4)-Ser(5)-Pro(6)-Ser(7) consensus repeats. Serines 2, 5, and 7 are known to be phosphorylated, and these modifications help to orchestrate the interplay between transcription and processing of messenger RNA (mRNA) precursors. Here, we provide evidence that phosphorylation of CTD Thr(4) residues is required specifically for histone mRNA 3' end processing, functioning to facilitate recruitment of 3' processing factors to histone genes. Like Ser(2), Thr(4) phosphorylation requires the CTD kinase CDK9 and is evolutionarily conserved from yeast to human. Our data thus illustrate how a CTD modification can play a highly specific role in facilitating efficient gene expression.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678764/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678764/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hsin, Jing-Ping -- Sheth, Amit -- Manley, James L -- R01 GM028983/GM/NIGMS NIH HHS/ -- R01 GM28983/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2011 Nov 4;334(6056):683-6. doi: 10.1126/science.1206034.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biological Sciences, Columbia University, New York, NY 10027, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22053051" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Cell Line
;
Cell Survival
;
Chickens
;
Cleavage And Polyadenylation Specificity Factor/metabolism
;
Cyclin-Dependent Kinase 9/metabolism
;
Histones/*genetics
;
Humans
;
Molecular Sequence Data
;
Nuclear Proteins/metabolism
;
Phosphorylation
;
*RNA 3' End Processing
;
RNA Polymerase II/chemistry/*metabolism
;
RNA, Messenger/*metabolism
;
Threonine/*metabolism
;
mRNA Cleavage and Polyadenylation Factors/metabolism
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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