Publication Date:
2000-06-02
Description:
Human granulocytic ehrlichiosis (HGE) is a febrile tick-borne illness caused by a recently discovered intracellular bacterium remarkable for its tropism for professionally phagocytic neutrophils. Monoclonal antibodies against the P-selectin binding domain of the leukocyte P-selectin glycoprotein ligand, PSGL-1, prevented HGE cell binding and infection, as did enzymatic digestion of PSGL-1. Furthermore, simultaneous neoexpression in nonsusceptible cells of complementary DNAs for both PSGL-1 and its modifying alpha-(1,3) fucosyltransferase, Fuc-TVII, allowed binding and infection by HGE. Thus, the HGE bacterium specifically bound to fucosylated leukocyte PSGL-1. Selectin mimicry is likely central to the organism's unique ability to target and infect neutrophils.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Herron, M J -- Nelson, C M -- Larson, J -- Snapp, K R -- Kansas, G S -- Goodman, J L -- R01AI40952/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2000 Jun 2;288(5471):1653-6.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Division of Infectious Diseases, Department of Medicine, University of Minnesota School of Medicine, Minneapolis, MN 55455, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10834846" target="_blank"〉PubMed〈/a〉
Keywords:
Antibodies, Monoclonal
;
B-Lymphocytes/microbiology
;
Cell Line
;
Ehrlichia/metabolism/*pathogenicity
;
Fluorescent Antibody Technique, Indirect
;
Fucosyltransferases/genetics/metabolism
;
Glycosylation
;
Granulocytes/metabolism/*microbiology
;
HL-60 Cells
;
Humans
;
Ligands
;
Membrane Glycoproteins/genetics/immunology/*metabolism
;
Metalloendopeptidases/metabolism
;
Molecular Mimicry
;
Neutrophils/metabolism/*microbiology
;
Oligosaccharides/genetics/immunology/metabolism
;
P-Selectin/metabolism
;
Transfection
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink