Publication Date:
1990-04-20
Description:
Ventricular myocytes exhibit a nifedipine-sensitive inward calcium current (ICa) and contracture when they are voltage clamped from -40 to 0 millivolt in the presence of caffeine and in the absence of extracellular sodium. However, upon repolarization they fail to relax because neither the sarcoplasmic reticulum nor the sodium-calcium exchange can reduce intracellular calcium. Sudden application of extracellular sodium during the contracture (but after repolarization) causes immediate relaxation and activates a transient inward sodium-calcium exchange current (INa-Ca), whose peak slightly precedes mechanical relaxation. The total charge carried by the nifedipine-sensitive ICa is twice the total charge carried by the transient inward INa-Ca. Assuming an exchange stoichiometry of three sodium to one calcium, these results indicate that all the calcium entering the cell during the initial depolarization is extruded by the sodium-calcium exchange.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bridge, J H -- Smolley, J R -- Spitzer, K W -- HL34288/HL/NHLBI NIH HHS/ -- HL42357/HL/NHLBI NIH HHS/ -- HL42873/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1990 Apr 20;248(4953):376-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah, Salt Lake City 84112.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/2158147" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Caffeine/pharmacology
;
Calcium/metabolism
;
Calcium Channels/*physiology
;
Carrier Proteins/*physiology
;
Electric Conductivity
;
Guinea Pigs
;
Heart/*physiology
;
Membrane Potentials
;
Myocardial Contraction/drug effects
;
Myocardium/metabolism
;
Nifedipine/pharmacology
;
Sarcoplasmic Reticulum/metabolism
;
Sodium/metabolism/pharmacology
;
Sodium-Calcium Exchanger
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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