Publication Date:
2010-05-04
Description:
Polycomb group (PcG) proteins are transcriptional repressors that control processes ranging from the maintenance of cell fate decisions and stem cell pluripotency in animals to the control of flowering time in plants. In Drosophila, genetic studies identified more than 15 different PcG proteins that are required to repress homeotic (HOX) and other developmental regulator genes in cells where they must stay inactive. Biochemical analyses established that these PcG proteins exist in distinct multiprotein complexes that bind to and modify chromatin of target genes. Among those, Polycomb repressive complex 1 (PRC1) and the related dRing-associated factors (dRAF) complex contain an E3 ligase activity for monoubiquitination of histone H2A (refs 1-4). Here we show that the uncharacterized Drosophila PcG gene calypso encodes the ubiquitin carboxy-terminal hydrolase BAP1. Biochemically purified Calypso exists in a complex with the PcG protein ASX, and this complex, named Polycomb repressive deubiquitinase (PR-DUB), is bound at PcG target genes in Drosophila. Reconstituted recombinant Drosophila and human PR-DUB complexes remove monoubiquitin from H2A but not from H2B in nucleosomes. Drosophila mutants lacking PR-DUB show a strong increase in the levels of monoubiquitinated H2A. A mutation that disrupts the catalytic activity of Calypso, or absence of the ASX subunit abolishes H2A deubiquitination in vitro and HOX gene repression in vivo. Polycomb gene silencing may thus entail a dynamic balance between H2A ubiquitination by PRC1 and dRAF, and H2A deubiquitination by PR-DUB.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182123/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3182123/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Scheuermann, Johanna C -- de Ayala Alonso, Andres Gaytan -- Oktaba, Katarzyna -- Ly-Hartig, Nga -- McGinty, Robert K -- Fraterman, Sven -- Wilm, Matthias -- Muir, Tom W -- Muller, Jurg -- R01 GM086868/GM/NIGMS NIH HHS/ -- R01 GM086868-13/GM/NIGMS NIH HHS/ -- RC2 CA148354/CA/NCI NIH HHS/ -- RC2 CA148354-02/CA/NCI NIH HHS/ -- RC2CA148354/CA/NCI NIH HHS/ -- England -- Nature. 2010 May 13;465(7295):243-7. doi: 10.1038/nature08966. Epub 2010 May 2.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/20436459" target="_blank"〉PubMed〈/a〉
Keywords:
Alleles
;
Animals
;
Biocatalysis
;
Drosophila Proteins/genetics/*metabolism
;
Drosophila melanogaster/embryology/*enzymology/genetics/metabolism
;
Gene Silencing
;
Genes, Homeobox/genetics
;
Genes, Insect/genetics
;
Genetic Complementation Test
;
Histones/*metabolism
;
Humans
;
Multiprotein Complexes/chemistry/isolation & purification/*metabolism
;
Nucleosomes/chemistry/metabolism
;
Polycomb Repressive Complex 1
;
Repressor Proteins/genetics/isolation & purification/*metabolism
;
Ubiquitin/metabolism
;
Ubiquitin Thiolesterase/chemistry/genetics/*metabolism
;
Ubiquitination/*physiology
Print ISSN:
0028-0836
Electronic ISSN:
1476-4687
Topics:
Biology
,
Chemistry and Pharmacology
,
Medicine
,
Natural Sciences in General
,
Physics
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