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  • 1
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    PLANT VOLATILES. Biosynthesis of monoterpene scent compounds in roses (2015)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2015-07-04
    Description: The scent of roses (Rosa x hybrida) is composed of hundreds of volatile molecules. Monoterpenes represent up to 70% percent of the scent content in some cultivars, such as the Papa Meilland rose. Monoterpene biosynthesis in plants relies on plastid-localized terpene synthases. Combining transcriptomic and genetic approaches, we show that the Nudix hydrolase RhNUDX1, localized in the cytoplasm, is part of a pathway for the biosynthesis of free monoterpene alcohols that contribute to fragrance in roses. The RhNUDX1 protein shows geranyl diphosphate diphosphohydrolase activity in vitro and supports geraniol biosynthesis in planta.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Magnard, Jean-Louis -- Roccia, Aymeric -- Caissard, Jean-Claude -- Vergne, Philippe -- Sun, Pulu -- Hecquet, Romain -- Dubois, Annick -- Hibrand-Saint Oyant, Laurence -- Jullien, Frederic -- Nicole, Florence -- Raymond, Olivier -- Huguet, Stephanie -- Baltenweck, Raymonde -- Meyer, Sophie -- Claudel, Patricia -- Jeauffre, Julien -- Rohmer, Michel -- Foucher, Fabrice -- Hugueney, Philippe -- Bendahmane, Mohammed -- Baudino, Sylvie -- New York, N.Y. -- Science. 2015 Jul 3;349(6243):81-3. doi: 10.1126/science.aab0696.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Laboratoire BVpam, EA3061, Universite de Lyon/Saint-Etienne, 23 Rue du Dr Michelon, F-42000, Saint-Etienne, France. ; Laboratoire BVpam, EA3061, Universite de Lyon/Saint-Etienne, 23 Rue du Dr Michelon, F-42000, Saint-Etienne, France. Laboratoire Reproduction et Developpement des Plantes UMR Institut National de la Recherche Agronomique (INRA)-CNRS, Universite Lyon 1-ENSL, Ecole Normale Superieure de Lyon, 46 Allee d'Italie, 69364 Lyon Cedex 07, France. ; Laboratoire Reproduction et Developpement des Plantes UMR Institut National de la Recherche Agronomique (INRA)-CNRS, Universite Lyon 1-ENSL, Ecole Normale Superieure de Lyon, 46 Allee d'Italie, 69364 Lyon Cedex 07, France. ; INRA, Institut de Recherche en Horticulture et Semences (INRA, AGROCAMPUS-OUEST, Universite d'Angers), SFR 4207 QUASAV, BP 60057, 49071 Beaucouze Cedex, France. ; Genomiques Fonctionnelles d'Arabidopsis, Unite de Recherche en Genomique Vegetale, UMR INRA 1165-Universite d'Evry Val d'Essonne-ERL CNRS 8196, Evry, France. ; INRA, Universite de Strasbourg, UMR 1131 Sante de la Vigne et Qualite du Vin, 28 Rue de Herrlisheim, F-68000 Colmar, France. ; Universite de Strasbourg-CNRS, UMR 7177, Institut Le Bel, 4 Rue Blaise Pascal, 67070 Strasbourg Cedex, France. ; INRA, Universite de Strasbourg, UMR 1131 Sante de la Vigne et Qualite du Vin, 28 Rue de Herrlisheim, F-68000 Colmar, France. sylvie.baudino@univ-st-etienne.fr philippe.hugueney@colmar.inra.fr mohammed.bendahmane@ens-lyon.fr. ; Laboratoire Reproduction et Developpement des Plantes UMR Institut National de la Recherche Agronomique (INRA)-CNRS, Universite Lyon 1-ENSL, Ecole Normale Superieure de Lyon, 46 Allee d'Italie, 69364 Lyon Cedex 07, France. sylvie.baudino@univ-st-etienne.fr philippe.hugueney@colmar.inra.fr mohammed.bendahmane@ens-lyon.fr. ; Laboratoire BVpam, EA3061, Universite de Lyon/Saint-Etienne, 23 Rue du Dr Michelon, F-42000, Saint-Etienne, France. sylvie.baudino@univ-st-etienne.fr philippe.hugueney@colmar.inra.fr mohammed.bendahmane@ens-lyon.fr.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/26138978" target="_blank"〉PubMed〈/a〉
    Keywords: Molecular Sequence Data ; Monoterpenes/*metabolism ; *Odors ; Plastids/*enzymology ; Pyrophosphatases/*biosynthesis/genetics ; Rosa/*enzymology/genetics ; Terpenes/*metabolism ; Transcriptome ; Volatile Organic Compounds/*metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    In situ detection of expression of thegus reporter gene in transgenic plants: ten years of blue genes (1996)
    Springer
    Details
    Publication Date: 1996-09-01
    Print ISSN: 0962-8819
    Electronic ISSN: 1573-9368
    Topics: Biology
    Published by Springer
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  • 3
    Electronic Resource
    Electronic Resource
    Direct regenerationin vitro and transient GUS expression inMentha xpiperita (1996)
    Springer
    Plant cell reports 16 (1996), S. 67-70 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic transformation of peppermint is known to be very difficult essentially because of low efficiency regeneration. A regeneration protocol allowing 51% shooting frequency is proposed. Transient β-glucuronidase expression and adjustment of selection pressure with kanamycin are also reported. The final retained method to attempt peppermint transformation is:Agrobacterium inoculation or biolistic treatment of the first apical leaves ofin vitro clones, regeneration in the dark with kanamycin (1 mg l−1) and 6-benzylaminopurine (2 mg l−1), followed by selection of regenerated shoots with 200 mg 1−1 kanamycin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275452
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  • 4
    Electronic Resource
    Electronic Resource
    A simple and efficient method for in vitro shoot regeneration from leaves of lavandin (Lavandula××intermedia Emeric ex Loiseleur) (1999)
    Springer
    Plant cell reports 18 (1999), S. 429-433 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsLavandula×intermedia ; Leaf explant ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A shoot regeneration system from leaves of lavandin (Lavandula×intermedia Emeric ex Loiseleur) was developed. The best results were obtained using a set of four different media. Callus was obtained on Murashige and Skoog (MS) medium containing 9 μm 6-benzylaminopurine and 4.5 μm α-naphthaleneacetic acid. After 2 weeks of culture, calli were transferred onto MS medium supplemented with 18 μm 6-benzylaminopurine to trigger bud regeneration. Shoot elongation was then stimulated by 1 μm gibberellin A3. Rooting was induced with 1 μm indole-3-butyric acid. All plantlets survived to greenhouse acclimatization. This is the first description of bud regeneration from leaves of lavandin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050598
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  • 5
    Electronic Resource
    Electronic Resource
    Direct regeneration in vitro and transient GUS expression in Mentha×piperita (1996)
    Springer
    Plant cell reports 16 (1996), S. 67-70 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations. BA: 6-benzylaminopurine; GUS: β-glucuronidase; MS: Murashige and Skoog (1962); NAA: α-naphthaleneacetic acid; PIG: particle inflow gun; SEM: scanning electron microscope.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. Genetic transformation of peppermint is known to be very difficult essentially because of low efficiency regeneration. A regeneration protocol allowing 51% shooting frequency is proposed. Transient β-glucuronidase expression and adjustment of selection pressure with kanamycin are also reported. The final retained method to attempt peppermint transformation is: Agrobacterium inoculation or biolistic treatment of the first apical leaves of in vitro clones, regeneration in the dark with kanamycin (1 mg l–1) and 6-benzylaminopurine (2 mg l–1), followed by selection of regenerated shoots with 200 mg l–1 kanamycin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050178
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  • 6
    Electronic Resource
    Electronic Resource
    Electron microscopy and X-ray microanalysis as tools for fine localization of the β-glucuronidase activity in transgenic plants harbouring the GUS reporter gene (1992)
    Springer
    Protoplasma 170 (1992), S. 68-76 
    Details
    ISSN: 1615-6102
    Keywords: β-Glucuronidase ; Cytoenzymology ; CaMV 35S-GUS ; Transgenic tobacco ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The GUS reporter gene encoding β-glucuronidase is very useful in various domains of plant genetic engineering. A method for ultrastructural detection of its activity was developed using 35S-GUS transgenic tobacco root tips. Short glutaraldehyde prefixation at 4°C preserved up to 70% enzyme activity and was followed by brief incubation in X-Glu, strong postfixations, then quick dehydration at low temperature before resin embedding. In these conditions, transgenic cells were well preserved and displayed electron dense indigo precipitates with a crystalline structure as shown by electron diffraction. Due to other dense structures in the tissues, controls of the nature of the reaction product (diX-indigo) were necessary. A first control was carried out by means of X-ray microanalysis in order to check the presence of bromine. Other controls, including incubated non-transformed tissues, non-incubated or boiled transgenic roots as well as transgenic samples incubated with the specific β-glucuronidase inhibitor, D-saccharic acid-1,4-lactone, were also carried out. The discussion points out the potential uses but also the limits of the method, non-specific localizations of the diX-indigo microcrystals being possible.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01384458
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  • 7
    Electronic Resource
    Electronic Resource
    Localization of target cells and improvement ofAgrobacterium-mediated transformation efficiency by direct acetosyringone pretreatment of carrot root discs (1993)
    Springer
    Protoplasma 174 (1993), S. 10-18 
    Details
    ISSN: 1615-6102
    Keywords: Acetosyringone ; Agrobacterium ; Carrot ; Cell cycle ; GUS assay ; Indole acetic acid levels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Localization of target cells forAgrobacterium-mediated transformation in the carrot root disc model has been achieved after inoculation with a disarmedA. tumefaciens strain harbouring a GUS-intron construct. The first GUS positive cells could be detected on both sides of the discs 48 h after inoculation. The transformed cells were always more numerous on the apical side, mainly localized in the intrafascicular cambium and in the immature phloem strands. The kinetics of free endogenous IAA levels on both sides after wounding have been determined, indicating that rapid IAA accumulation on the apical side was not simply due to polar migration from the basal side. Attempts to optimize transformation efficiency were made by pretreating the discs with various concentrations of acetosyringone (AS) and/or naphthalene acetic acid (NAA). Surprisingly, while 25 μM AS applied to bacteria prior to the inoculations was ineffective, the same AS concentration applied as a pretreatment to the discs strongly increased the number of transformed cells in the target tissues and decreased the lag time for the appearance of the first GUS positive cells. NAA pretreatment on the basal side enhanced the AS effect. AS pretreatment was found both to advance the reentry of competent cells with a potential for cell division into the S phase of the cell cycle and to stimulate bacterial attachment to the cell walls. The relationship between transformation efficiency and DNA synthesis in the host cells is discussed. AS treatment of plant tissues prior to inoculation is proposed as a means of increasing the transformation rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01404037
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  • 8
    Electronic Resource
    Electronic Resource
    In situ detection of expression of thegus reporter gene in transgenic plants: ten years of blue genes (1996)
    Springer
    Transgenic research 5 (1996), S. 281-288 
    Details
    ISSN: 1573-9368
    Keywords: reporter genes ; β-glucuronidase ; transgenic plants ; immunocytochemistry ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Among the methods now available to localize the sites of gene expression in plant materials, reporter genes based on thegus (uidA) gene ofEscherichia coli, which encodes a β-glucuronidase (E.C. 3.2.1.31; GUS), have been the most widely used during the last ten years. The apparent simplicity of the histochemical GUS assay has been a major factor in the increase in articles usinggus genes. However, over the last four years, there have been occasional reports expressing doubts concerning the specificity of the observed localizations based on discrepancies between results obtained with GUS histochemistry and immunocytochemistry and/orin situ hybridization. This brief review compares the results obtained with immunocytochemistry with those obtained with various GUS substrates for histochemical studies. Certain sources of artefact are discussed, as are the limits that should be imposed on interpretation of GUS histochemistry results at the organ, tissue and cell levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968938
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