Publication Date:
2004-06-05
Description:
Strategies for inhibiting phagolysosome fusion are essential for the intracellular survival and replication of many pathogens. We found that the lysosomal synaptotagmin Syt VII is required for a mechanism that promotes phagolysosomal fusion and limits the intracellular growth of pathogenic bacteria. Syt VII was required for a form of Ca2+-dependent phagolysosome fusion that is analogous to Ca2+-regulated exocytosis of lysosomes, which can be triggered by membrane injury. Bacterial type III secretion systems, which permeabilize membranes and cause Ca2+ influx in mammalian cells, promote lysosomal exocytosis and inhibit intracellular survival in Syt VII +/+ but not -/- cells. Thus, the lysosomal repair response can also protect cells against pathogens that trigger membrane permeabilization.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Roy, Deepannita -- Liston, David R -- Idone, Vincent J -- Di, Anke -- Nelson, Deborah J -- Pujol, Celine -- Bliska, James B -- Chakrabarti, Sabyasachi -- Andrews, Norma W -- AI34867/AI/NIAID NIH HHS/ -- AI43389/AI/NIAID NIH HHS/ -- AI48507/AI/NIAID NIH HHS/ -- GM64625/GM/NIGMS NIH HHS/ -- New York, N.Y. -- Science. 2004 Jun 4;304(5676):1515-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Section of Microbial Pathogenesis and Department of Cell Biology, Yale University School of Medicine, New Haven, CT 06510, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15178804" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Bacteria/*growth & development/metabolism
;
Bacterial Proteins/genetics/metabolism
;
CHO Cells
;
Calcium/metabolism
;
*Calcium-Binding Proteins
;
Cell Membrane/*physiology
;
Cells, Cultured
;
Cricetinae
;
Endocytosis
;
Exocytosis
;
Listeria monocytogenes/growth & development
;
Lysosomes/microbiology/physiology
;
Macrophages/microbiology
;
Membrane Glycoproteins/genetics/*physiology
;
Mice
;
Mutation
;
Nerve Tissue Proteins/genetics/*physiology
;
Permeability
;
Phagosomes/microbiology/physiology
;
Salmonella typhimurium/*growth & development/metabolism
;
Synaptotagmins
;
Vacuoles/microbiology
;
Yersinia pseudotuberculosis/genetics/growth & development
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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