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  • 1
    Electronic Resource
    Electronic Resource
    Quantitative histological studies of compatible and incompatible interactions between accessions of Macroptilium atropurpureum and Uromyces appendiculatus (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 37 (1988), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Quantitative histological studies of pre-penetration, penetration and post-penetration events were made for one compatible interaction and five interactions showing varying degrees of incompatibility between Uromyces appendiculatus and Macroptilium atropurpureum. Four histologically different resistant responses to infection were observed, CPI 91348 exhibited a response, characterized by significantly fewer pustules per unit area and a longer generation time than the susceptible cultivar Siratro. Significantly fewer urediniospores produced appressoria on 91348 than on Siratro. Resistance in CPI 84997 was associated with the development of large areas of necrotic tissue; 99.8% of colonies showed an indeterminant hypersensitive response. Colony areas for the above three host genotypes were not significantly different at 144 h after inoculation.The remaining lines developed varying degrees of chlorosis only. Colonies in CQ 1382 and (CPI 92643 grew only slowly, but were still growing 144 h after inoculation and, as with CPI 84997, this growth continued after necrotic tissue appeared (indeterminant hypersensitivity), In CQ 1398, colony growth ceased by 96 144 h after inoculation, coinciding with the appearance of necrotic tissue (determinant hypersensitivity).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1988.tb02200.x
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  • 2
    Electronic Resource
    Electronic Resource
    Etiology of a newly described root rot of guar (Cyamopsis tetragonoloba) in Australia caused by Phytophthora cryptogea (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 35 (1986), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: An isolate of Phytophthora obtained from diseased roots and stems of guar (Cyamopsis tetragonoloba) growing in waterlogged soil from Dalby. Queensland. was identified as Phytophothora cryptogea (P. drechsleri). Sex organs were not observed, Pathogenicity to guar seedlings was established under glasshouse conditions either by using a hypocotyl-wound inoculation technique, or adding motile zoospores to seedlings growing under saturated conditions. This appears to be the first report of this disease. Seven isolates of P. drechsleri from hosts other than guar were shown to be pathogenic to guar following hypocotyls-wound inoculation.The host range of the guar isolate was studied using both hypocotyl-wound inoculation and zoospores. After hypocotyl inoculation, the following species were found to be susceptible: cantaloupe. cowpea, cucumber, mungbean, pea. pigeon-pea, safflower. sunflower, tomato and watermelon. Slight symptoms were observed on bean and chickpea. When zoospores were used as the inoculum, only pea. pigeon-pea, sunflower and safflower were highly susceptible. A range of guar cultivars and breeding lines were screened for resistance using zoospore inoculum, and although no genotypes were highly resistant, there were significant differences between disease reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1986.tb02051.x
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  • 3
    Electronic Resource
    Electronic Resource
    Glasshouse and field evaluation of cowpea lines for partial resistance to Phytophthora vignae (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 43 (1994), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The reaction of cowpea lines to naturally occurring Phytophthora vignae in the field was studied in three trials over the period 1987–89 with the aim of identifying and characterizing lines with partial resistance. Seven lines (CPI 84853, CPI 84851, CPI 65393, CPI 77359, CPI 78209. CPI 78213 and cultivar Blackeye 5) were found to be partially resistant. Partial resistance was assessed by calculating disease incidence, disease severity, time lag before onset of disease, relative area under the disease progress curve and apparent mortality and infection rates. Significant differences in some of the characters further divided the partially resistant lines into different groups. It was concluded that the characters found to be most useful in the differentiation of relative levels of partial resistance were final percentage infection, and final percentage of plants with non-killing lesions. There were also significant correlations between these variables within and between years, although the relative rankings of some of the lines changed between years. Overall, CPI 84853 and CPI 65393 showed higher levels of partial resistance in the field than the remaining lines. In general, the partially resistant lines gave variable reactions following hypocotyl inoculation with P. vignae. There were clear indications of host-isolate interactions, suggesting that partial resistance may be no more durable than complete resistance for this particular host-pathogen interaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1994.tb00548.x
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  • 4
    Electronic Resource
    Electronic Resource
    Aetiology of stem canker diseases of pigeonpea in Fiji (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 38 (1989), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Xanthomonas campestris pv. cajani is reported for the first time from Fiji as the cause of potentially serious pustular stem lesions of pigeonpea. Cultivars and breeding lines differed in susceptibility. The cause of another stem canker disease, characterized by smooth lesions, remains undetermined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1989.tb02165.x
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  • 5
    Electronic Resource
    Electronic Resource
    Quantitatively expressed resistance to anthracnose (Colletotrichum gloeosporioides) in Stylosanthes scabra (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Plant pathology 37 (1988), S. 0 
    Details
    ISSN: 1365-3059
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Nineteen accessions of Stylosanthes scabra that had low terminal severities of anthracnose in the field were evaluated for their response in the glasshouse using four isolates representing three different races of Colletotrichum gloeosporioides. Accessions could be allocated to one of three groups: one showed a higher disease severity after inoculation with isolate UQ14 compared to the other isolates; the second showed a higher severity after inoculation with isolates WRS32 and/or WRS20 compared to isolates SR4 and UQ14, and the third group showed moderate to low disease severities following inoculation with all four isolates. Based on reduced disease severity and compatible infection types, a set of six accessions was selected from the three groups for further testing, two of which showed no significant difference in their response to the four isolates. Two components of resistance, incubation period and infection efficiency were studied in the selected accessions. Of these, incubation period appeared unrelated to the expression of resistance. Criteria for identifying this form of quantitatively expressed resistance are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3059.1988.tb02111.x
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  • 6
    Electronic Resource
    Electronic Resource
    The Experimental and Commercial Release of Transgenic Crop Plants (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 111 (1993), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: With advances in recombinant DNA methods and transformation procedures, it is possible to transfer genes into crop plants from unrelated plants, microbes and animals. Many of the modifications being carried out, or envisaged, are for disease and pest resistance, product quality and tolerance to environmental stress, but there are additional opportunities to modify crops to give specialized products for industrial or pharmaceutical use. Some of the characteristics of transgenic plants are considered, including: transgene copy number, position, expression, stability, pleiotropy, selectable marker genes and somaclonal variation. There have been several hundreds of field trials with transgenic plants, and the first transgenic varieties are likely to be approved for commercial production in 1993. Before releasing transgenic plants, it is necessary to carry out a risk assessment to determine whether the transgenic variety will behave differently from a conventionally bred variety. Assessment procedures are being harmonized internationally by various organizations. There is a growing commitment to apply these genetic modification methods to crops in developing countries, as genes relevant to their crops and environments become available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1993.tb00602.x
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  • 7
    Electronic Resource
    Electronic Resource
    CgT1: a non-LTR retrotransposon with restricted distribution in the fungal phytopathogenColletotrichum gleosporioides (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 320-331 
    Details
    ISSN: 1617-4623
    Keywords: Retroposon ; Colletotrichum gloeosporioides ; Anthracnose DNA fingerprinting ; Horizontal transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two genetically distinct biotypes (A and B) ofColletotrichum gloeosporioides that cause different anthracnose diseases on the legumesStylosanthes spp. have been identified in Australia. A DNA sequence that was present in biotype B and absent in biotype A was isolated by differential hybridisation of a genomic library using total genomic DNA of each biotype as hybridisation probes. This sequence also failed to hybridise to DNA of three biotypes ofC. gloeosporioides from other host species and to DNA of three other species ofColletotrichum. This clone was used to isolate two cosmid clones of biotype B. Sequence analysis of these clones revealed a repetitive element of approximately 5.7 kb in length. This element, termedCgT1, was dispersed in the genome and present in about 30 copies. The element contained open reading frames encoding deduced sequence motifs homologous togag-like proteins, reverse transcriptase and RNase H domains of non-LTR retrotransposons. The termini ofCgT1 lacked long terminal repeats (LTRs) but contained a 3′ A-rich domain. The insertion site of one copy of the element was flanked by short 13-bp direct repeats. These characteristics of the termini, taken together with the overall structure and sequence homologies, indicate thatCgT1 belongs to the non-LTR, LINE-like retrotransposon class of elements that are present in many eukaryotes. PCR primers designed to amplify regions ofCgT1 can be used to distinguish biotypes A and B in Australia. DNA fingerprinting analysis of genomic DNA using hybridisation probes derived from the terminal regions ofCgT1 revealed that Australian isolates of biotype B are monomorphic.CgT1 was not detected in some isolates causing Type B disease from other countries and whenCgT1 was present there was considerable polymorphism inCgT1 organisation in the genome.CgT1 is the first transposon-like element to be identified in the genusColletotrichum and has considerable potential as a tool for the study of population structure, genome dynamics and evolution inC. gloeosporioides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02173778
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  • 8
    Electronic Resource
    Electronic Resource
    Evidence for outcrossing in Phytophthora sojae and linkage of a DNA marker to two avirulence genes (1994)
    Springer
    Current genetics 27 (1994), S. 77-82 
    Details
    ISSN: 1432-0983
    Keywords: Phytophthora megasperma f. sp. glycinea ; Avirulence ; Gene-for-gene ; RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two genetically different isolates of the homothallic Oomycete, Phytophthora sojae, were demonstrated to outcross and form hybrid oospores after co-culturing in vitro. Random amplified polymorphic DNA (RAPD) markers revealed ten hybrids among 354 oospores analysed. One F1 hybrid was allowed to self fertilise and produce an F2 population of 247 individuals. Among 53 F2 individuals, selected at random, 18 polymorphic RAPD markers were observed to segregate at near 3:1 Mendelian ratios, consistent with segregation for dominant alleles at single loci. Segregation of virulence against soybean resistance genes Rps1a, 3a, and 5 revealed that the avirulence genes Avr1a, 3a and 5 were dominant to virulence. Avirulence against these three resistance genes appeared to be conditioned by one locus for Avr1a and two independent, complementary dominant loci for both Avr3a and Avr5. Segregation of virulence against Rps6 was in the ratio of 1:2:1 (avirulent:mixed reaction:virulent), suggesting a semi-dominant allele at a single locus. Two avirulence genes and one RAPD marker formed one linkage group, in the order Avr3a, OPH4-1, Avr5, each separated by approximately 5 cM. Our results confirm that outcrossing occurred between the parental isolates, and that sexual recombination under field conditions may play an important role in generating and maintaining genetic diversity in populations of P. sojae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326582
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  • 9
    Electronic Resource
    Electronic Resource
    The use of bulk segregant analysis to identify a RAPD marker linked to leaf rust resistance in barley (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 270-273 
    Details
    ISSN: 1432-2242
    Keywords: Barley ; Puccinia hordei ; Resistance genes ; Bulk segregant analysis ; Polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An F2 population from a cross between barley accession Q21861 and the Australian barley variety ‘Galleon’ was used to develop RAPD markers for resistance to barley leaf rust (Puccinia hordei). Resistant and susceptible DNA bulks were constructed following the classification of F2 plants by leaf rust infection type. Bulked segregant analysis was then used to identify a 2.7-kb marker, designated OU022700 and located approximately 12cM from RphQ, a leaf rust resistance gene in Q21861. The marker was generated by PCR with the oligonucleotide primer OPU-02 (Operon). Infection types of F3 progeny were used to confirm assignment of F2 genotypes. OU022700 was shown, retrospectively, to be useful in the identification of individual F2 plants that had been originally misclassified as having susceptible infection types. Both the RAPD marker and RphQ will be potentially useful in the development of new barley cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220888
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  • 10
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    Remarkable species diversity in Malagasy mouse lemurs (primates, Microcebus) (2000)
    National Academy of Sciences
    Details
    Publication Date: 2000-09-26
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
    Published by National Academy of Sciences
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