ISSN:
1573-4838
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
,
Technology
Notes:
Abstract The technique of microcalorimetry has been extensively applied in studies of metabolic processes in human blood cells in suspension. In contrast to other types of blood cells, human granulocytes and other phagocytic cells are activated by contact with the stainless steel vessel in which the sample under study is enclosed. The activation is characterized by the production of increased quantities of heat energy with variable time-dependent profiles. The present study demonstrates that lining the stainless steel vessels with fluoroethylene-polypropylene will completely eliminate the contact activation of granulocytes. It is shown that this modified procedure enhances the quality of microcalorimetry as a technique for the measurement of granulocyte metabolism and function under physiological conditionsin vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00705284
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