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    Arabidopsis galactolipid biosynthesis and lipid trafficking mediated by DGD1 (1999)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1999-06-26
    Description: The photosynthetic apparatus in plant cells is associated with membranes of the thylakoids within the chloroplast and is embedded into a highly specialized lipid matrix. Diacylglycerol galactolipids are common in thylakoid membranes but are excluded from all others. Isolation of the gene DGD1, encoding a galactosyltransferase-like protein, now provides insights into assembly of the thylakoid lipid matrix and subcellular lipid trafficking in Arabidopsis thaliana.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dormann, P -- Balbo, I -- Benning, C -- New York, N.Y. -- Science. 1999 Jun 25;284(5423):2181-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biochemistry, Michigan State University, East Lansing, MI 48824, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10381884" target="_blank"〉PubMed〈/a〉
    Keywords: Arabidopsis/genetics/growth & development/*metabolism ; *Arabidopsis Proteins ; Base Sequence ; Chloroplasts/metabolism ; Chromosome Mapping ; DNA, Complementary/genetics ; Endoplasmic Reticulum/metabolism ; Exons ; Galactolipids ; Galactosyltransferases/chemistry/*genetics/*metabolism ; Genes, Plant ; Glycolipids/*biosynthesis ; Intracellular Membranes/metabolism ; *Lipid Metabolism ; Molecular Sequence Data ; Mutation ; Plants, Genetically Modified ; Recombinant Proteins/metabolism
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
    Electronic Resource
    Electronic Resource
    Comparison of four radioactive probes for the diagnosis of potato spindle tuber viroid by nucleic acid spot hybridization (1988)
    Springer
    Potato research 31 (1988), S. 431-442 
    Details
    ISSN: 1871-4528
    Keywords: PSTV detection ; DNA-RNA probes ; RNA transcription ; nick-translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary 32P-labelled probes prepared by nick-translation, two methods (A and B) of labelling PSTV cDNA in M13 phage DNA, and RNA transcription were compared for their sensitivity in detecting PSTV. Nick-translated and method B probes were the least sensitive. The minimum concentration of PSTV detected by these probes was 100 pg. Method A probe detected 10–20 pg of PSTV whereas RNA transcripts were capable of detecting as little as 0.33 pg of PSTV. RNA transcripts are easy to prepare, and their use in hybridization analysis do not cause background reactions. Sometimes low non-specific signals are produced but they can be eliminated by washing membranes with RNase A. RNA probes are now used for routine detection of PSTV in tuber flesh and sprouts, leaves and true seed of potato at the International Potato Center (CIP).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02357880
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