ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The Agrobacterium radiobacter CCRC 14924 N-carbamyl-D-amino-acid amidohydrolase, the enzyme used for production of D-amino acids, was overexpressed in Escherichia coli JM109. The expressed protein was crystallized by vapour diffusion using lithium sulfate as precipitant. It crystallizes in space group P21 with unit-cell parameters a = 69.8, b = 67.9 and c = 137.8 Å and β = 96.4°. There are four molecules per asymmetric unit. Crystals diffract to 2.8 Å resolution using a rotating-anode source at cryogenic (113 K) temperatures.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S090744499801525X
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