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  • 1
    Electronic Resource
    Electronic Resource
    17β-estradiol in vitro affects Na-dependent and depolarization-induced Ca2+ transport in rat brain synaptosomes (1996)
    Springer
    Cellular and molecular life sciences 52 (1996), S. 217-220 
    Details
    ISSN: 1420-9071
    Keywords: Estradiol ; calcium transport ; synaptosomes ; brain ; rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Effects of 17β-estradiol (E2) in vitro on Na-dependent Ca2+ efflux from, and depolarization-induced Ca2+ uptake into, the nerve cell were studied with the use of synaptosomes isolated from the brain stem, mesencephalic reticular formation (MRF), caudate nucleus and the hippocampus of long-term ovariectomized adult female rats. It was found that E2 (1) at a concentration of 10 nM or lower, stimulates Na-dependent Ca2+ efflux in the caudate nucleus and hippocampus, and does not affect the efflux in MRF and brain stem; (2) at concentrations above 10 nM has no effect on the Ca2+ efflux in any of the four structures investigated; and (3) produces a biphasic effect on the depolarization-induced Ca2+ uptake, increasing it in all structures except MRF at 10 nM concentration, and decreasing it at concentrations higher than 10 nM, irrespective of the structure investigated. These results suggest that E2, acting at extranuclear sites, modulates synaptic transmission via alterations of Ca2+ transport mechanisms in nerve endings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01920709
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  • 2
    Electronic Resource
    Electronic Resource
    Cellular binding sites for insulin in rat liver
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 382 (1975), S. 609-620 
    Details
    ISSN: 0005-2736
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2736(75)90226-6
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  • 3
    Electronic Resource
    Electronic Resource
    Enhanced detection of glycoproteins in polyacrylamide gels
    Amsterdam : Elsevier
    Analytical Biochemistry 170 (1988), S. 491-494 
    Details
    ISSN: 0003-2697
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-2697(88)90663-X
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  • 4
    Electronic Resource
    Electronic Resource
    Ca^2^+ uptake by rat brain region synaptosomes following in vivo administration of ovarian steroids
    Amsterdam : Elsevier
    Molecular and Cellular Endocrinology 57 (1988), S. 77-80 
    Details
    ISSN: 0303-7207
    Keywords: Brain stem ; Calcium uptake ; Estradiol ; Hippocampus ; Mesencephalic reticular formation ; Nucleus caudatus ; Progesterone ; Synaptosome
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0303-7207(88)90034-2
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  • 5
    Electronic Resource
    Electronic Resource
    Insulin binding by a cell line (HT 29) derived from human colonic cancer
    Amsterdam : Elsevier
    Molecular and Cellular Endocrinology 14 (1979), S. 123-130 
    Details
    ISSN: 0303-7207
    Keywords: insulin degradation ; receptors ; target cells ; tissue culture ; transformed cells
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0303-7207(79)90100-X
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  • 6
    Electronic Resource
    Electronic Resource
    Biochemical characterization of the lysosomes of ehrlich ascites tumor cells
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 148 (1967), S. 725-740 
    Details
    ISSN: 0304-4165
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-4165(67)90046-3
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  • 7
    Electronic Resource
    Electronic Resource
    The occurrence and intracellular localization of sucrase activity in rat liver
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 297 (1973), S. 413-422 
    Details
    ISSN: 0304-4165
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-4165(73)90088-3
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  • 8
    Electronic Resource
    Electronic Resource
    Evidence for suppression of Na-dependent Ca2+ efflux from rat brain synaptosomes by ovarian steroids in vivo (1991)
    Springer
    Cellular and molecular life sciences 47 (1991), S. 623-625 
    Details
    ISSN: 1420-9071
    Keywords: Calcium uptake ; synaptosomes ; rat brain ; progesterone ; estradiol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The possibility that intracellular Ca2+, which mediates neurotransmitter release, regulation of membrane permeability, microtubule polymerization and axonal transport, is influenced by gonadal steroids via a Na−Ca exchange mechanism was examined. The resting Ca2+ uptake into synaptosomes was measured using crude synaptosomal pellets (P2 fraction), isolated from the brain stem, mesencephalic reticular formation (MRF), nucleus caudatus (NC) and the hippocampus of intact, long-term ovariectomized (OVX) and OVX plus progesterone (P) or estradiol-17β benzoate (EB) treated adult female rats. Irrespective of the brain structure investigated, the uptake was 1) markedly increased in synaptosomes from OVX animals in comparison to intact controls, and 2) reduced to near control values in synaptosomes from OVX rats treated s.c. with a single dose of 2 mg P or 5 μg EB. Since Ca2+ influx into synaptosomes was shown earlier to depend on external sodium concentration, which was the same in all experiments described in this work, the results obtained indicate that ovarian steroids modulate basal synaptic activity in the rat brain by suppressing Na-dependent Ca2+ efflux from the nerve cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01949890
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  • 9
    Electronic Resource
    Electronic Resource
    Interactive Sorption of Metal Ions and Humic Acids onto Mineral Particles (1999)
    Springer
    Water, air & soil pollution 111 (1999), S. 41-56 
    Details
    ISSN: 1573-2932
    Keywords: effects of pH ; humic acids ; metal ions ; mineral particles ; sorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The sorption of metal ions (Pb2+, Zn2+ and Cu2+) and soil humic acids (HA) from aqueous solutions onto mineral particles (sand, calcite and clay) was investigated using a batch equilibrium system. The sorption reactions in two- component systems (heavy metals-mineral particles and humic acids- mineral particles), as well as interactions in three-component system (heavy metals-humic acids-mineral particles) were examined. Results showed that the presence of humic acids, dissolved or bound onto mineral surfaces, considerably influenced the fixation of heavy metals. The various effects, depending on mineral type, humic concentration and specific metal-ion, were observed in three- component system. Sorption of Cu2+-ions on all minerals studied rapidly increased as the concentration of dissolved HA increased. The amount of Pb2+-ions sorbed on sand slightly decreased, while on kaolin increased between 15 and 20%. Sorption of Zn2+-ions on all minerals studied decreased at pH 4. At pH 5.5 the sorption of Zn2+-ions onto calcite decreased, while on kaolin and sand increased as a function of the humic acid concentration giving the curve with maximum at c(HA) = 2.5 mmol C L-1. At pH 6.5 sorption onto kaolin and sand increased. This effect occurs as a result of the conditional stability constant of Zn-HA complexes increasing at higher pH which in turn promotes the chelation of Zn2+-ions to mineral- bound humic substances. The enhanced sorption of metal ions from the aqueous phase in three-component systems is not only the result of mineral sorption of free metals but also the result of chelation with HA sorbed on the mineral surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005084802830
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  • 10
    Electronic Resource
    Electronic Resource
    Photometrische Kationenbestimmung mit Gelatinefolien (1975)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 273 (1975), S. 101-102 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Kationen, Kupfer ; Spektralphotometrie ; Gelatinefolien
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Eine einfache und schnelle Methode zur photometrischen Kationenbestimmung mittels Gelatinefolien wurde ausgearbeitet. Eine reine Filmfolie als Träger der Farbreaktion wird in die Kationenlösung und danach in die Reagenslösung eingetaucht, wobei sich in der Gelatineschicht ein gefärbter Niederschlag bildet, dessen Farbintensität proportional der Metallkonzentration ist. Die Folie wird photometriert und mittels einer Eichkurve die Metallkonzentration bestimmt. Das Verfahren wurde an Hand der Cu-Bestimmung mit Diäthyldithiocarbamidat erprobt. Die Färbung befolgt in diesem Fall das Beersche Gesetz. Eine Bestimmung erfordert etwa 20 min. Der relative Fehler beträgt ±5 % bei einer Konzentration von 3–30 μg Cu2+/ml.
    Notes: Abstract A simple and rapid method for the photometric determination of cations using a strip of gelatin film has been developed. The strip is immersed in the sample solution and afterwards in a reagent solution. The intensity of a the coloured precipitate formed in the strip is proportional to the metal ion concentration, which is determined photometrically using a calibration curve. The determination of Cu with diethyldithiocarbamidate has been selected for testing the technique. The colour intensity in this case follows Beer's law. The determination can be performed within 20 min. The relative error amounts to ±5 % for a concentration range of 3–30 μg of Cu2+/ml.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00426266
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