ISSN:
1573-4986
Keywords:
α(2-6)-sialyltransferase
;
CMP-Neu5Ac
;
Galβ1-4GlcNAc α(2-6)-N-acetylneuraminyltransferase
;
sialyloligosaccharides
;
1H-NMR spectroscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract Incubation of synthetic Man\1-4GlcNAcβ-OMe, GalNAcβ1-4GlcNAcβ-OMe, Glcβ1-4GlcNAcβ-OMe, and GlcNAcβ1-4GlcNacβ-OMe with CMP-Neu5Ac and rat liver Galβ1-4GlcNAc α(2-6)-sialyltransferase resulted in the formation of Neu5Acα2-6Manβ1-4GlcNAcβ-OMe, Neu5Acα2-6GalNAcβ1-4GlcNAcβ-OMe, Neu5Acα2-6Glcβ1-4GlcNAcβ-OMe and Neu5Acα2-6GlcNAcβ1-4GlcNAcβ-OMe, respectively. Under conditions which led to quantitative conversion of Galβ1-4GlcNAcβ-OEt into Neu5Acα2-6Galβ1-4GlcNAcβ-OEt, the aforementioned products were obtained in yields of 4%, 48%, 16% and 8%, respectively. HPLC on Partisil 10 SAX was used to isolate the various sialyltrisaccharides, and identification was carried out using 1- and 2-dimensional 500-MHz1H-NMR spectroscopy.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00731191
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