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  • 1
    Electronic Resource
    Electronic Resource
    Structural studies of the interaction between indole derivatives and biologically important aromatic compounds. XIII. Ring stacking interaction on the thiamin-tryptophan systems (1985)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 107 (1985), S. 3305-3314 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00297a041
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  • 2
    Electronic Resource
    Electronic Resource
    Short term retinol treatment in vitro induces stable transdifferentiation of chick epidermal cells into mucus-secreting cells (1991)
    Springer
    Development genes and evolution 200 (1991), S. 289-295 
    Details
    ISSN: 1432-041X
    Keywords: Retinol ; Hydrocortisone ; Mucous metaplasia ; Transdifferentiation ; Epidermis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Epidermal mucous metaplasia of cultured skin can be induced by treatment with excess retinol for several days (Fell 1957). In the induction of mucous metaplasia, retinol primarily affects the dermal cells and retinol-pretreated dermis can alter epidermal differentiation towards secretory epithelium (Obinata et al. 1987). In this work, we found that mucous metaplasia could be induced by culturing 13-day-old chick embryonic tarsometatarsal skin in medium containing retinol (20 μM) for only 8–24 h, followed by culture in a chemically defined medium (BGJb) without retinol or serum for 6 days. The application of cycloheximide together with retinol during the first 8 h of culture inhibited epidermal mucous metaplasia during subsequent culture for 6 days in BGJb, indicating that induction of a signal(s) in the dermis by excess retinol requires protein synthesis. However, the presence of 20 nM hydrocortisone (Takata et al. 1981) throughout the culture period did not inhibit retinol-induced epidermal mucous metaplasia of the epidermis. This indicates that a brief treatment of the skin with excess retinol determines the direction of epithelial differentiation toward secretory epithelium; this is a simpler in vitro system for the induction of epidermal mucous metaplasia than those established before.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00241298
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  • 3
    Electronic Resource
    Electronic Resource
    Intracellular binding of cationized ferritin prolongs the time course of sodium channel inactivation in squid giant axons (1986)
    Springer
    The journal of membrane biology 89 (1986), S. 75-83 
    Details
    ISSN: 1432-1424
    Keywords: sodium channel ; inactivation ; cationized ferritin ; cytoplasmic surface ; surface charge ; anionic sites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary Cationized ferritin (CF) applied intracellularly in squid giant axons bound to the negatively charged sites on the cytoplasmic surface of the axolemma. Under the electron microscope, the distribution of CF was found to be dense and uniform over the axolemmal surface. However, the effect of CF on the membrane excitability was highly specific, the major effect being a prolonging of the inactivation time course of the sodium channel without altering the properties of the potassium channel. The binding of CF did not alter the surface potential related to the membrane excitability. When CF was present intracellularly, the time course of the inactivation was characterized by two time constants (slow and fast). The slow component increased with an increase in CF binding and its time constant had a unique value (26 msec) irrespective of the duration of perfusion and concentration of CF. The concentration of CF at which the half-maximum response occurred was about 150nm. Poly-l-glutamate, charged negatively at neutral pH, removed CF from the axolemma and counteracted the CF effect on the sodium channel, although this poly-acidper se did not affect the membrane excitability. Our results indicate that CF binds electrostatically to the inactivation site of the sodium channel but does not affect the voltage sensor, which is supposed to be located deep in the channel.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01870897
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  • 4
    Electronic Resource
    Electronic Resource
    Correlation of betacyanin synthesis with cell division in cell suspension cultures of Phytolacca americana (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 90 (1994), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: In suspension cultures of Phytolacca americana, betacyanin accumulation was reduced when cell division was inhibited by treatment with various inhibitors of DNA synthesis or anti-microtubule drugs. Aphidicolin (APC), an inhibitor of DNA synthesis, reduced the incorporation of radioactivity from labeled tyrosine into betacyanin, but the incorporation of radioactivity from labeled 3,4-dihydroxyphenylalanine (DOPA) into betacyanin was not affected by similar treatments. Propyzamide, another anti-microtubule drug, reduced incorporation of radioactivity from tyrosine and DOPA into betacyanin. However, the rate of incorporation from DOPA was higher than that from tyrosine. The results suggest that inhibition of betacyanin accumulation in Phytolacca americana cells by APC and propyzamide is due to suppression of the reaction converting tyrosine to DOPA, which may be closely related to cell division.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1994.tb00383.x
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  • 5
    Electronic Resource
    Electronic Resource
    Stimulation by 2,4-dichlorophenoxyacetic acid of betacyanin accumulation in suspension cultures of Phytolacca americana (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 83 (1991), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: 2,4-Dichlorophenoxyacetic acid (2,4-D) strongly promoted betacyanin accumulation in suspension cultures of Phytolacca americana L. The betacyanin accumulation attained a maximum at 5 μM 2,4-D, when betacyanin content per cell reached 252% as compared to the control (2,4-D free). 2,4-D elevated the level of free tyrosine, which is the precursor of betacyanin. The addition of 1 mM tyrosine to the medium partially reversed the reduction of betacyanin accumulation caused by the removal of 2,4-D. Tracer experiments using labelled tyrosine showed that 2,4-D activated the biosynthetic pathway from tyrosine to betacyanin. These results indicate that a sufficient supply of tyrosine and the activation of biosynthesis of betacyanin from tyrosine by 2,4-D elevate the level of betacyanin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1991.tb01295.x
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  • 6
    Electronic Resource
    Electronic Resource
    The new lead citrate method for the ultracytochemical demonstration of activity of non-specific alkaline phosphatase (orthophosphoric monoester phosphohydrolase) (1967)
    Springer
    Histochemistry and cell biology 11 (1967), S. 88-96 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The new method, utilizing “lead citrate” as capture reagent, for the ultracytochemical demonstration of non-specific alkaline phosphatase activity at high alkaline ranges of pH was introduced. Results obtained by the new “lead citrate” method in various tissues of the rat were presented and discussed in connection with observations made by other methods. The method can be used at the light microscopic level also.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00326615
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  • 7
    Electronic Resource
    Electronic Resource
    Ultracytochemical demonstration of cholinesterase activity in the atrium of the guinea-pig heart (1969)
    Springer
    Histochemistry and cell biology 17 (1969), S. 49-56 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural distribution of cholinesterase (ChE) activity was examined in the atrium of the guinea-pig heart using Karnovsky's method. The reaction products showing the ChE activity were found mainly in the neural elements in the atrium. There were axons showing no reaction products intermingled with axons showing the positive enzymatic activity. In the enzymatically positive axons, the reaction products were observed in the axon-Schwann interspace, the interspace between neighboring axons and the corresponding plasma membranes in nerve fiber bundles as well as preterminal axons. It was of interest to find that not only axons containing ordinary agranular vesicles, but also axons with larger granular vesicles showed the ChE activity. In terminals the enzymatic activity was found also in the axon-Schwann interspace, the interspace between the axolemma and the sarcolemma and the corresponding plasma membranes of the enzymatically positive areas. In the neuron the enzymatic activity was positive in the endoplasmic reticulum and the interspace between the neuronal plasma membrane and the corresponding plasma membrane of the satellite cell. Plasma membranes facing to these enzymatically positive spaces were also positive for the enzymatic activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306329
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  • 8
    Electronic Resource
    Electronic Resource
    Regulatory mechanisms of biosynthesis of betacyanin and anthocyanin in relation to cell division activity in suspension cultures (1994)
    Springer
    Plant cell, tissue and organ culture 38 (1994), S. 167-169 
    Details
    ISSN: 1573-5044
    Keywords: Anthocyanin ; betacyanin ; cell division ; Phytolacca ; suspension culture ; Vitis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regulatory mechanisms of betacyanin biosynthesis in suspension cultures of Phytolacca americana and anthocyanin in Vitis sp. were investigated in relation to cell division activity. Betacyanin biosynthesis in Phytolacca cells clearly shows a positive correlation with cell division, as the peak of betacyanin accumulation was observed at the log phase of batch cultures. Incorporation of radioactivity from labelled tyrosine into betacyanin also showed a peak at early log phase. Aphidicolin, an inhibitor of DNA synthesis, and propyzamide, an antimicrotubule drug, reduced betacyanin accumulation and inhibited the incorporation of radioactivity from labelled tyrosine into betacyanin at concentrations which were inhibitory to cell division. Both inhibitors reduced the incorporation of radioactivity from labelled tyrosine to 3,4-dihydroxyphenylalanine (DOPA), but the incorporation of labelled DOPA into betacyanin was not affected. These results suggest that the conversion of tyrosine to DOPA is coupled with cell division activity. In contrast, the anthocyanin accumulation in Vitis cells showed a negative correlation with cell division. Accumulation occurred at the stationary phase in batch cultures when cell division ceased. Aphidicolin or reduced phosphate concentration induced a substantial increase in anthocyanin accumulation as well as the inhibition of cell division. Chalcone synthase (CHS) activity increased at the time of anthocyanin accumulation. Northern blotting analysis indicated that changes in CHS mRNA levels corresponded to similar changes in enzymatic activity. The pool size of endogenous phenylalanine was low during active cell division, but increased before anthocyanin began to accumulate and concomitantly with increasing levels of CHS mRNA. Exogenous supply of phenylalanine at the time of low endogenous levels induced the elevation of CHS mRNA and anthocyanin accumulation. These results indicate that the elevation of endogenous phenylalanine levels, when cell division ceases, may cause the increase in CHS mRNA levels, resulting in increased CHS activity and subsequently in anthocyanin accumulation in Vitis suspension cultures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00033874
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  • 9
    Electronic Resource
    Electronic Resource
    Detection of a glycosphingolipid antigen in bladder cancer cells with monoclonal antibody MRG-1 (1995)
    Springer
    Journal of molecular histology 27 (1995), S. 247-252 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The monoclonal antibody MRG-1 has been evaluated for the immunohistochemical detection of the type 3 chain of blood group A in human normal bladder epithelium and bladder tumours. Light microscope examination of paraffin sections demonstrated that this antigen was present in normal epithelium and superficial bladder tumour in patients with blood group A or AB, but was absent in the invasive type of bladder tumour. In normal epithelium, the plasma membrane was positive for this antigen, and the cytoplasm was diffusely stained. In superficial transitional cell carcinoma, the plasma membrane was negative, whereas the cytoplasm was intensely stained in the perinuclear region. This pattern was different from that observed for type 1 and 2 group A antigen, which was recognized mainly at the plasma membrane. However, in superficial transitional cell carcinoma, the staining was also seen on the plasma membrane. The pattern of the localization of this antigen in this carcinoma was influenced by the treatment of organic solvents. Electron microscopical observations confirmed that this antigen was localized on the plasma membrane and also in the Golgi apparatus of the superficial tumour. These results proved that the type 3 chain of blood group A is present in human bladder epithelium and low grade tumours in correspondence with the blood type, but disappears in tumours with high malignant potential. However, its expression is independent of the expressions of the other subtypes which have been studied. Furthermore, the changes in the staining pattern caused by pretreatment with organic solvents suggested possible differences in the microenvironment of the glycolipids containing this type of sugar chain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00177592
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  • 10
    Electronic Resource
    Electronic Resource
    Detection of a glycosphingolipid antigen in bladder cancer cells with monoclonal antibody MRG-1 (1995)
    Springer
    Journal of molecular histology 27 (1995), S. 247-252 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The monoclonal antibody MRG-1 has been evaluated for the immunohistochemical detection of the type 3 chain of blood group A in human normal bladder epithelium and bladder tumours. Light microscope examination of paraffin sections demonstrated that this antigen was present in normal epithelium and superficial bladder tumour in patients with blood group A or AB, but was absent in the invasive type of bladder tumour. In normal epithelium, the plasma membrane was positive for this antigen, and the cytoplasm was diffusely stained. In superficial transitional cell carcinoma, the plasma membrane was negative, whereas the cytoplasm was intensely stained in the perinuclear region. This pattern was different from that observed for type 1 and 2 group A antigen, which was recognized mainly at the plasma membrane. However, in superficial transitional cell carcinoma, the staining was also seen on the plasma membrane. The pattern of the localization of this antigen in this carcinoma was influenced by the treatment of organic solvents. Electron microscopical observations confirmed that this antigen was localized on the plasma membrane and also in the Golgi apparatus of the superficial tumour. These results proved that the type 3 chain of blood group A is present in human bladder epithelium and low grade tumours in correspondence with the blood type, but disappears in tumours with high malignant potential. However, its expression is independent of the expressions of the other subtypes which have been studied. Furthermore, the changes in the staining pattern caused by pretreatment with organic solvents suggested possible differences in the microenvironment of the glycolipids containing this type of sugar chain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02389892
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