ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Two-dimensional gel (2-D) electrophoresis coupled to fluorography was used to obtain the [35S]-methionine labeled protein profiles of cultured human retinal pigment epithelial cells. These patterns revealed about 250 polypeptides in the largely acidic isoelectric focusing 2-D profile and approximately 200 in the mainly basic non-equilibrium pH gradient electrophoresis 2-D profile. The isolation of a detergent-resistant cytoskeletal preparation revealed an enriched content of intermediate-sized (7-10 nm) filaments as shown by electron microscopy. Two-dimensional analysis (acidic isoelectric focusing, 2-D) of the cytoskeletal preparation showed about 30 major proteins including non-muscle actin, which had their counterparts in the total retinal pigment epithelium protein profile. These studies have begun to establish the subcellular local (filaments) and identity of some of the currently visualized 450 different retinal pigment epithelium proteins.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150040206
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