ISSN:
0173-0835
Schlagwort(e):
Chemistry
;
Biochemistry and Biotechnology
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
When [3H]-labeled proteins are analyzed by polyacrylamide gel electrophoresis, the sensitivity of fluorograhic detection following gel impregnation with PPO (2,5-diphenyloxazole) in DMSO (dimethylsulfoxide) is significantly reduced if the gel has been stained with Coomassie Brilliant Blue R-250. Since the reduction in fluorographic efficiency depends on the concentration of stain, which in turn depends upon protein concentration, substantial errors may enter into quantitative measurements of fluorograms depending on sample composition. Another reduction in fluorographic efficiency results from reduced penetrability of the gels to PPO when a build-up of methanol in DMSO and DMSO/PPO solutions is allowed. This loss of fluorographic sensitivity should be sample independent, however.
Zusätzliches Material:
2 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/elps.1150030407
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