ISSN:
1573-4927
Keywords:
gene transfer
;
liposome
;
IGF-I
;
wound healing
;
rat
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Our hypothesis is that gene transfer of an IGF-I CMV-cDNA with cholesterol containing cationic liposomes is an efficient tool for transient transfection of growth factors in vitro and in vivo. In vitro, we transiently cotransfected IGF-I cDNA with a CMV construct and a Lac Z CHKβCHK-galactosidase cDNA/CMV construct using cholesterol containing cationic liposomes and measured CHKβCHK-galactosidase and IGF-I mRNA and protein. In vivo, we subcutaneously injected 3-month-old male Sprague–Dawley rats with IGF-I cDNA and CHKβCHK-galactosidase cDNA into rat skin. After IGF-I and CHKβCHK-galactosidase were cotransfected into PC12 cells, Northern blot analysis showed that the peak time of IGF-I expression was 2 days for mRNA and 5 days for protein. In vivo, a cDNA/liposome ratio of 1:2 was most effective. IGF-I protein expression in IGF-I-transfected skin resulted in significant transfection from day 5 to day 7. In situ determination of CHKβCHK-galactosidase activity confirmed that transfections resulted in a restricted expression area.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1001880220039
Permalink