ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract l-[4,5-3H]isoleucine was introduced to label anteiso-fatty acid (AIFA)-containing lipids in Staphylococcus aureus SG 511. After an overnight incubation in peptone broth in the presence of 37 kBq l-[4,5-3H]isoleucine/ml, 8.5–13% of the total radioactivity applied was found to be incorporated into the cells. 22.4–25.6% of the incorporated radioactivity was found in AIFA-containing lipids extracted by chloroform-methanol-water (2:1:02, v/v/v) at pH 2. The interphase contained 70–75% of the incorporated radioactivity. Lipoteichoic acid, extracted by phenol-water 80:20, w/v) contained less than 1% of the incorporated radioactivity, as measured after purification by hydrophobic interaction chromoatography on octyl sepharose gel. Within 1 h after addition of 10μg/ml penicillin G to exponentially growing cultures of S. aureus, that led to non-lytic death of the cells, 11.9–18.1% of the incorporated l-[4,5-3H] isoleucine label were released. Lipids containing AIFA were excreted to 5.4–8.4% of total incorporated activity; this amount represents more than 1/4 of the labeled cellular lipids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1990.tb03790.x
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