ISSN:
1662-9752
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Superficial bladder cancer is often treated by removing the cancerous portion of thebladder wall combined with immuno-chemotherapy; in more extreme cases, it is often necessary toremove the entire bladder wall. This diagnosis brings an obvious need for bladder tissue replacementdesigns with a high degree of efficacy. Since bladder cells are accustomed to interacting withextracellular matrix proteins having dimensions on the nanometer scale, this study aimed to designthe next generation of tissue-engineered bladder replacement constructs with nanometer (less than100 nm) surface features. For this purpose, porous and biodegradable PLGA and PU scaffolds weretreated with various concentrations of NaOH or HNO3, respectively, for various periods of time tocreate nanometer surface roughness. Resulting surface properties were characterized using SEM (tovisualize scaffold properties) and BET. Cell experiments conducted on these polymeric scaffoldsprovided evidence of enhanced bladder smooth muscle cell attachment, growth, and elastin/collagenproduction (critical extracellular matrix proteins in the bladder tissue regeneration process) as surfacefeature dimensions were reduced into the nanometer regime. In vivo augmentation surgeries withnano-structured PLGA and PU patches will provide further information regarding total bladdercapacity, anastomotic integrity, burst pressure, epithelialization, muscular ingrowth, andneovascularization. In vitro and in vivo proof of material usefulness and technique would provideurologists with a readily accessible graft for bladder tissue replacement applications
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/02/14/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.539-543.540.pdf
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