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  • 1
    Electronic Resource
    Electronic Resource
    The pattern of carbon fixation in the marine unicellular alga Phaeodactylum tricornutum (1976)
    Springer
    Marine biology 38 (1976), S. 189-199 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The short- and long-term fixation of 14CO2 by Phaeodactylum tricornutum was studied using methods of fractionation that allowed examination of all the products labelled with 14C. There was no doubt that the major pathway of CO2 fixation was into 3-phosphoglycerate, but there was also significant incorporation by β-carboxylation by means of phosphoenol-pyruvate carboxylase and transamination into aspartate. At short time intervals (10 sec), 90% of the radioactive products found were accounted for by 3-phosphoglycerate and aspartate. The lipids associated with the photosynthetic apparatus contained a high proportion of the 14C fixed, which at 60 sec was located mainly in the carbohydrate portion of the lipids. At 30 and 300 min, the chlorophylls, carotenoids and the long-chain fatty acids were heavily labelled with 14C. The monogalacto-diglycerides, the digalacto-diglycerides and the sulpholipids each had a characteristic long-chain fatty acid composition. The cell proteins and a reserve polysaccharide were also labelled with 14C at short time intervals and increased their radioactivity in a linear fashion up to the longest period studied, 300 min. The activity of the enzymes ribulose diphosphate carboxylase and phosphoenolpyruvate carboxylase was sufficient to account for the pattern of fixation found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388932
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of vitamin D on enzyme activities in the mucosal cells of the chick small intestine (1970)
    Springer
    The journal of membrane biology 3 (1970), S. 43-53 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary A search was made for enzyme activities that are increased after vitamin D treatment of rachitic chicks. Three enzyme activities located in the brush borders of the mucosal cells of the intestine — ATPase, p-nitrophenyl phosphatase, and pyrophosphatase — were found to approximately double in activity 48 hr after vitamin D was given. The ATPase and the p-nitrophenyl phosphatase required Mg++ for activity but could be further stimulated by addition of Ca++. The three activities are probably caused by the same enzyme since 20 mM phenylalanine inhibited all three activities. It is unlikely that the Ca++-stimulated ATPase is concerned with Ca++ translocation since phenylalanine, which inhibits this enzyme, had no effect on45Ca transport from mucosal to serosal fluids of everted sacs of intestine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01868005
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  • 3
    Electronic Resource
    Electronic Resource
    New evidence for the role of cyclic AMP in the release of mitochondrial calcium (1980)
    Springer
    The journal of membrane biology 52 (1980), S. 185-186 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01869125
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  • 4
    Electronic Resource
    Electronic Resource
    Absorption of Vitamin B 12 from the Rat Intestine (1956)
    [s.l.] : Nature Publishing Group
    Nature 177 (1956), S. 701-702 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To study the importance of 'binding' in the absorption of vitamin B12, it was necessary to use animals deprived of that portion of their gut where binding substances are located. The following technique was devised to allow the retention of a small amount of labelled vitamin B12 in a section of the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/177701a0
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  • 5
    Electronic Resource
    Electronic Resource
    A Cyanocobalamin–Protein Complex from Sow's Milk and Desiccated Pig Stomach (1954)
    [s.l.] : Nature Publishing Group
    Nature 173 (1954), S. 830-830 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The pink protein complex from sow's milk has the absorption spectrum shown in Fig. 1. The absorption at 278 mjz is largely due to protein (EJ(l%9 lcm.) = 15-1) and at 362 mjx to cyanocobalamin (E(l%, 1 cm.) = 5*7). The total nitrogen (micro-Kjeldahl) is 16-1 per cent, and 1 mgm. of the complex ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/173830a0
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  • 6
    Electronic Resource
    Electronic Resource
    Vitamin B 12 and Protein Biosynthesis: Vitamin B 12 and Biosynthesis in Chick Liver (1959)
    [s.l.] : Nature Publishing Group
    Nature 183 (1959), S. 519-523 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] IN recent years evidence has been produced that the steps of incorporation of amino-acids into proteins include: (i) an activation dependent on adenosine triphosphate of the amino-acids through the COOH group, with the formation of an amino-acid-adenosine monophosphate complex by means of enzymes ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/183519a0
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  • 7
    Electronic Resource
    Electronic Resource
    Reversal of Penicillin Inhibition by a Yeast Extract (1949)
    [s.l.] : Nature Publishing Group
    Nature 164 (1949), S. 314-315 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE preparation from yeast of a growth-factor for Corynebacterium diphtheriœ1,2 has shown the active concentrates to be complex mixtures containing amino-acids and peptides, one of the latter yielding on hydrolysis serine, glycine and glutamic acid. Attempted removal of the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/164314a0
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  • 8
    Electronic Resource
    Electronic Resource
    Differentiation of Vitamin B 12 Active Compounds by Ionophoresis and Microbiological Assay: Ionophoresis (1953)
    [s.l.] : Nature Publishing Group
    Nature 171 (1953), S. 148-149 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SINCE the isolation of vitanvn B12 and other members of this group, several other compounds have been detected, related to but not in the cobalamin. series. Wijmenga1 isolated from pig faeces a crystalline substance he called vitamin B12m; Ford and Porter2 found vitamin B12-like factors A, B and C ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/171148a0
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  • 9
    Electronic Resource
    Electronic Resource
    Differentiation of Vitamin B 12 Active Compounds by Ionophoresis and Microbiological Assay: Occurrence of the Various Vitamin B 12 Active Compounds (1953)
    [s.l.] : Nature Publishing Group
    Nature 171 (1953), S. 150-151 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] THE ionophoretic and microbiological findings just described show that compounds formerly accepted as pure were not, in fact, pure. We interpret our findings as proof that factor A and vitamin B12TO are not pure substances, but that they contain the same major component. Similarly, pseudo-vitamins ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/171150a0
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  • 10
    Electronic Resource
    Electronic Resource
    Effect of growth factors and light quality on the growth, pigmentation and photosynthesis of two diatoms, Thalassiosira gravida and Phaeodactylum tricornutum (1985)
    Springer
    Marine biology 86 (1985), S. 253-262 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When Thalassiosira gravida Cleve was grown at low photon-flux densities, its requirements of soil extract for growth could be partially replaced by a mixture of 10 mgl-1 humic acid, 10-7 M gibberellic acid and 5×10-7 M kinetin. The effect of these growth factors was to decrease the lag-phase and increase the number of cells without any qualitative or quantitative change in the pigments associated with photosynthesis. Phaeodactylum tricornutum Bohlin also produced more cells when grown in the presence of these growth factors, although this diatom can be subcultured without soil extract. An improved culture vessel allowed investigations of the two diatoms at low light intensities and illuminated with equal photon-flux densities of white light or blue light (maximum transmission 470 to 520 nm). Growth was always greater on the blue light, but the alteration of chlorophyll levels reported in the literature was not observed. Chlorophyll levels increased during the growth phase, whereas photosynthetic ability reached its maximum on the third or fourth day. The effects of growth factors and blue light on growth were additive and the cells produced had a photosynthetic activity greater than any previously reported. The superiority of equal quanta of blue light over white light in producing more cells can be explained as due to proportionally more quanta in blue light being absorbed by the photosynthetic pigments and thus providing more energy for growth and synthesis. A photosynthetic 14CO2-fixation action spectrum for P. tricornutum showed a maximum in the violet around 451 nm, and a second peak in the red around 678 nm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397512
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