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  • 1
    Publication Date: 2009-09-18
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Denker, Hans-Werner -- England -- Nature. 2009 Sep 17;461(7262):341. doi: 10.1038/461341b.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19759600" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cell Dedifferentiation ; Cloning, Organism/*ethics/legislation & jurisprudence/methods/*trends ; Embryo Research/ethics/legislation & jurisprudence ; Fibroblasts/cytology ; Humans ; Mice ; Pluripotent Stem Cells/cytology
    Print ISSN: 0028-0836
    Electronic ISSN: 1476-4687
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Comparative Biochemistry and Physiology -- Part B: Biochemistry and 99 (1991), S. 709-712 
    ISSN: 0305-0491
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Analytical Biochemistry 152 (1986), S. 39-41 
    ISSN: 0003-2697
    Keywords: immobilized fluorescein-labeled gelatin ; proteinase assay
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Developmental Biology 126 (1988), S. 203-211 
    ISSN: 0012-1606
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Experimental Cell Research 215 (1994), S. 40-50 
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 39 (1974), S. 193-193 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 38 (1974), S. 331-338 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An improved variant of the protease substrate film test is presented which combines high sensitivity and more accurate localization while allowing control of the chemical purity of the substrate. Directions for the preparation of gelatin films are given in detail, critical steps being the fixation and swelling in alkaline solution. Post-incubation staining rather than the use of prestained films is preferred to avoid inhibition of enzymes. Poststaining also makes histologic details easily discernible. The substrate films are suitable for routine use. They have been applied specifically for the investigation of sperm acrosomal proteases and blastocyst proteases; the result of these tests are also briefly described.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 21 (1970), S. 17-20 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei der Verwendung von perjodsäureoxydierten Stärkefilmen (Shear und Pearse, 1963) zum Amylasenachweis können Artefakte entstehen durch alkalische, nicht-enzymatische Hydrolyse in Geweben mit hohem pH. Dies wird anhand von Beobachtungen an Kaninchen-Blastozysten erläutert.
    Notes: Summary In substrate film tests for amylase, periodate-oxidized starch films (Shear and Pearse, 1963) may produce artifacts, for they undergo spontaneous hydrolysis at slightly alkaline pH levels. This is concluded from observations made on rabbit blastocysts.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 25 (1971), S. 256-267 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung An Frühstadien der Entwicklung des Kaninchens von der Ovulation bis zur Implantation (einschließlich der umgebenden mütterlichen Gewebe) wird die Topochemie von Enzymen des Glykogenstoffwechsels untersucht. Für den Phosphorylase-Nachweis kommt eine neue methodische Variante zur Anwendung. Furchungsstadien sind gekennzeichnet durch eine hohe Aktivität in Keim und Tubenepithel. Beim Übergang in das Blastozytenstadium scheint der Glykogenabbau von besonderer Bedeutung zu sein. Gegen den Zeitpunkt der Implantation beginnt, gemessen an Phosphorylaseaktivität und Glykogenkonzentration, erneut ein reger Glykogenstoffwechsel, jetzt im Uterusepithel. Glykogensynthetase war in den hier interessierenden Strukturen nicht nachweisbar. Eine eventuelle Bedeutung von Amylase wird diskutiert (Tubenepithel, Blastozystenhöhle).
    Notes: Summary The early stages of development from the ovulation to implantation (including the Fallopian tube and uterus) are studied in the rabbit. The distribution of enzymes of the metabolism of glycogen is demonstrated. For phosphorylase, a new method is used. A high activity is found in the epithelium of the Fallopian tube and in the egg during cleavage. In blastocyst formation, the degradation of glycogen seems to be important, whereas phosphorylase activity in the epithelium of the uterus at the time of implantation is correlated with a storage of glycogen. Glycogen synthetase could not be demonstrated in these tissues. The possibility of a physiologic role of amylase is discussed.
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Histochemistry and cell biology 25 (1971), S. 268-285 
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Untersucht werden Frühstadien der Entwicklung des Kaninchens von der Ovulation bis zur Implantation, einschließlich Tube und Uterus. Registriert wird die Verteilung von Glykosidasen (β-Galaktosidase, Neuraminidase, Glukosaminidase, β-Glukuronidase, α-Glukosidase), vor allem im Hinblick auf den Stoffwechsel von Mukosubstanzen. Die verwendete Methode zum Neuraminidasenachweis ist ein erster Versuch zur histochemischen Lokalisation dieses Enzyms. Ergebnisse. In Furchungsstadien sind β-Glukuronidase und Glukosaminidase auffällig aktiv. Eine Funktion bei den wichtigen morphogenetischen Prozessen dieser Phase wird vermutet. Das Epithel der Tube zeigt vor allem eine Aktivität von Glukosaminidase und β-Galaktosidase, die möglicherweise eine Beziehung zur Bildung der Mukoproteidschicht haben. Im Uterusepithel sind in allen Stadien β-Galaktosidase und Glukosaminidase aktiv; β-Glukuronidase tritt vor allem vor dem Eintritt des Keims in den Uterus und bei der Implantation hervor. Für die Auflösung der Blastozystenhüllen und für die Implantation ist wahrscheinlich die β-Galaktosidase-, β-Glukuronidase-und Glukosaminidaseaktivität der Trophoblastsprosse von Bedeutung. Neuraminidase ist dagegen vor allem im Uterusepithel lokalisiert.
    Notes: Summary Early stages of development and the surrounding tissues of the Fallopian tube and the uterus are studied in the rabbit from ovulation to implantation. The topochemistry of the following glycosidases is demonstrated: β-galactosidase, neuraminidase, glucosaminidase, β-glucuronidase, α-glucosidase. The distribution given for neuraminidase is the result of preliminary attempts to develop histochemical procedures for this enzyme. Results. In cleaving eggs, the activities of β-glucuronidase and glucosaminidase are dominant. The epithelium of the Fallopian tube shows an activity of glucosaminidase and β-galactosidase possibly correlated to the formation of the mucoproteid layer. These enzymes are also demonstrated in the epithelium of the uterus in all stages, whereas β-glucuronidase has its maximum activity before eggs enter the uterus and at implantation. In the trophoblastic knobs, these three enzymes could have a physiological role in the processes of dissolution of blastocyst coverings and implantation. Neuraminidase activity is found in the epithelium of the uterus.
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