Publication Date:
1996-12-20
Description:
Recombination activating genes (RAG-1 and RAG-2), involved in V(D)J rearrangement of immunoglobulin genes, have been thought to be expressed only in immature stages of B-cell development. However, RAG-1 and RAG-2 transcripts were found to be reexpressed in mature mouse B cells after culture with interleukin-4 in association with several different co-stimuli. Reexpression was also detected in draining lymph nodes from immunized mice. RAG-1 and RAG-2 proteins could be detected by immunofluorescence microscopy in the nuclei of B cells cultured in vitro and in the germinal centers of draining lymph nodes. These findings suggest that RAG gene products play a heretofore unsuspected role in mature B cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hikida, M -- Mori, M -- Takai, T -- Tomochika, K -- Hamatani, K -- Ohmori, H -- New York, N.Y. -- Science. 1996 Dec 20;274(5295):2092-4.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Biotechnology, Faculty of Engineering, Okayama University, Tsushima-Naka, Okayama 700, Japan. hit2224@cc.okayama-u.ac.jp〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/8953042" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
B-Lymphocytes/immunology/*metabolism
;
Cell Nucleus/metabolism
;
Cells, Cultured
;
*DNA-Binding Proteins
;
*Gene Expression
;
*Genes, RAG-1
;
Germinal Center/metabolism
;
*Homeodomain Proteins
;
Immunoglobulin Class Switching
;
Interleukin-4/pharmacology
;
Interleukins/pharmacology
;
Lipopolysaccharides/pharmacology
;
Lymph Nodes/metabolism
;
*Lymphocyte Activation
;
Mice
;
Mice, Inbred C3H
;
Microscopy, Fluorescence
;
Protein Biosynthesis
;
Proteins/*genetics
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
Permalink