Publication Date:
1999-02-26
Description:
The alpha subunit (Gsalpha) of the stimulatory heterotrimeric guanosine triphosphate binding protein (G protein) Gs activates all isoforms of mammalian adenylyl cyclase. Adenylyl cyclase (Type V) and its subdomains, which interact with Gsalpha, promoted inactivation of the G protein by increasing its guanosine triphosphatase (GTPase) activity. Adenylyl cyclase and its subdomains also augmented the receptor-mediated activation of heterotrimeric Gs and thereby facilitated the rapid onset of signaling. These findings demonstrate that adenylyl cyclase functions as a GTPase activating protein (GAP) for the monomeric Gsalpha and enhances the GTP/GDP exchange factor (GEF) activity of receptors.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Scholich, K -- Mullenix, J B -- Wittpoth, C -- Poppleton, H M -- Pierre, S C -- Lindorfer, M A -- Garrison, J C -- Patel, T B -- HL48308/HL/NHLBI NIH HHS/ -- HL59679/HL/NHLBI NIH HHS/ -- New York, N.Y. -- Science. 1999 Feb 26;283(5406):1328-31.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pharmacology, University of Tennessee, Health Science Center, 874 Union Avenue, Memphis, TN 38163, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10037603" target="_blank"〉PubMed〈/a〉
Keywords:
Adenylyl Cyclases/chemistry/genetics/*metabolism
;
Animals
;
Cell Line
;
GTP Phosphohydrolases/*metabolism
;
GTP-Binding Protein alpha Subunits, Gs/*metabolism
;
GTPase-Activating Proteins
;
Guanine Nucleotide Exchange Factors
;
Guanosine 5'-O-(3-Thiotriphosphate)/metabolism
;
Guanosine Triphosphate/metabolism
;
Magnesium/pharmacology
;
Proteins/metabolism
;
Receptors, Adrenergic, beta-2/metabolism
;
Recombinant Proteins/metabolism
;
*Signal Transduction
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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