Publication Date:
2011-10-25
Description:
Inhibitor of apoptosis (IAP) proteins are negative regulators of cell death. IAP family members contain RING domains that impart E3 ubiquitin ligase activity. Binding of endogenous or small-molecule antagonists to select baculovirus IAP repeat (BIR) domains within cellular IAP (cIAP) proteins promotes autoubiquitination and proteasomal degradation and so releases inhibition of apoptosis mediated by cIAP. Although the molecular details of antagonist-BIR domain interactions are well understood, it is not clear how this binding event influences the activity of the RING domain. Here biochemical and structural studies reveal that the unliganded, multidomain cIAP1 sequesters the RING domain within a compact, monomeric structure that prevents RING dimerization. Antagonist binding induces conformational rearrangements that enable RING dimerization and formation of the active E3 ligase.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Dueber, Erin C -- Schoeffler, Allyn J -- Lingel, Andreas -- Elliott, J Michael -- Fedorova, Anna V -- Giannetti, Anthony M -- Zobel, Kerry -- Maurer, Brigitte -- Varfolomeev, Eugene -- Wu, Ping -- Wallweber, Heidi J A -- Hymowitz, Sarah G -- Deshayes, Kurt -- Vucic, Domagoj -- Fairbrother, Wayne J -- P41RR001209/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 2011 Oct 21;334(6054):376-80. doi: 10.1126/science.1207862.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Early Discovery Biochemistry, Genentech, 1 DNA Way, South San Francisco, CA 94080, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22021857" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Cell Line
;
Cell Line, Tumor
;
Cloning, Molecular
;
Humans
;
Hydrophobic and Hydrophilic Interactions
;
Inhibitor of Apoptosis Proteins/*antagonists & inhibitors/*chemistry/metabolism
;
Mice
;
Models, Biological
;
Models, Molecular
;
Molecular Sequence Data
;
Mutagenesis, Site-Directed
;
Proteasome Endopeptidase Complex/metabolism
;
Protein Conformation
;
Protein Interaction Domains and Motifs
;
Protein Multimerization
;
Protein Structure, Secondary
;
Scattering, Small Angle
;
Ubiquitin-Protein Ligases/chemistry/metabolism
;
Ubiquitinated Proteins/chemistry/metabolism
;
Ubiquitination
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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