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  • 1
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    Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay (2014)
    Oxford University Press
    Details
    Publication Date: 2014-08-15
    Description: A sensitive and highly multiplex method to directly measure RNA sequence abundance without requiring reverse transcription would be of value for a number of biomedical applications, including high throughput small molecule screening, pathogen transcript detection and quantification of short/degraded RNAs. R NA A nnealing, S election and L igation (RASL) assays, which are based on RNA template-dependent oligonucleotide probe ligation, have been developed to meet this need, but technical limitations have impeded their adoption. Whereas DNA ligase-based RASL assays suffer from extremely low and sequence-dependent ligation efficiencies that compromise assay robustness, Rnl2 can join a fully DNA donor probe to a 3'-diribonucleotide-terminated acceptor probe with high efficiency on an RNA template strand. Rnl2-based RASL exhibits sub-femtomolar transcript detection sensitivity, and permits the rational tuning of probe signals for optimal analysis by massively parallel DNA sequencing (RASL-seq). A streamlined Rnl2-based RASL-seq protocol was assessed in a small molecule screen using 77 probe sets designed to monitor complex human B cell phenotypes during antibody class switch recombination. Our data demonstrate the robustness, cost-efficiency and broad applicability of Rnl2-based RASL assays.
    Print ISSN: 0305-1048
    Electronic ISSN: 1362-4962
    Topics: Biology
    Published by Oxford University Press
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  • 2
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    Controlling Cotton's Insect Pests: A New System (1982)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1982-04-02
    Description: Cotton is more heavily treated with insecticides than any other crop in the United States. In southern Texas, this heavy treatment resulted in insecticide- resistant strains of major pests which almost destroyed the industry in the late 1960's and early 1970's. An integrated insect control program based on new short-season cotton varieties and traditional cultural practices has restored production in the area. The new system has been widely implemented because it produces greater net returns by reducing the use of insecticides, fertilizer, and irrigation.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Adkisson, P L -- Niles, G A -- Walker, J K -- Bird, L S -- Scott, H B -- New York, N.Y. -- Science. 1982 Apr 2;216(4541):19-22.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17809777" target="_blank"〉PubMed〈/a〉
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 3
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    Diffusion-zoned pyroxenes in an isotopically heterogeneous mantle lithosphere beneath the Dunedin Volcanic Group, New Zealand, and their implications for intraplate alkaline magma sources (2017)
    Geological Society of America (GSA)
    Details
    Publication Date: 2017-05-24
    Description: An important task in assessing the magma source of an intraplate volcanic province is establishing the composition of the underlying lithospheric mantle. Pyroxenes in peridotite xenoliths from the ~10,000 km 2 Dunedin Volcanic Group in New Zealand reveal that the underlying lithospheric mantle is chemically and isotopically heterogeneous and has a complex thermal history. Portions of this mantle have light rare earth element–depleted clinopyroxene trace element concentrations and distinctly radiogenic Nd compositions (Nd (20 Ma) ≥ + 15.5) with model depleted mantle ages that are ≥100 m.y. older than the overlying Jurassic crust (type 1). The Nd isotopic composition of these moderately fertile domains is distinct from any Dunedin Volcanic Group magma, but the domains are embedded within enriched peridotitic mantle (type 2) that has formed through reaction with a light rare earth element–rich fluid that imparted an isotopic composition in strongly metasomatized xenoliths of 87 Sr/ 86 Sr (20 Ma) = 0.7028–0.7029, Nd (20 Ma) = + 5.0 to + 5.1, and 206 Pb/ 204 Pb = 19.9, 207 Pb/ 204 Pb = 15.5, and 208 Pb/ 204 Pb = 39.6. These isotope ratios overlap with the isotopically homogeneous high time-integrated U/Pb–like source signature of the host Dunedin Volcanic Group. However, all metasomatic and nonmetasomatic pyroxenes are zoned in temperature-sensitive elements (Al, Cr, Mg ± Ca), with trends indicating element exchange during cooling and the results of diffusion calculations implying that the zoning formed over hundreds of thousands to millions of years prior to late Oligocene–Miocene xenolith entrainment. These data, along with calculated pyroxene rare earth element homogenization diffusion rates, indicate that mantle metasomatism predated entrainment in the host magmas by millions of years. Furthermore, the presence of the cooling trends in all but one sample indicates that this upper lithosphere mantle preserves little or no sign of a rise in the geotherm at the time of magmatism. Zoning patterns in peridotite pyroxenes can therefore provide useful insight into the role of portions of the lithospheric mantle in formation of intraplate alkaline basalts.
    Print ISSN: 1941-8264
    Electronic ISSN: 1947-4253
    Topics: Geosciences
    Published by Geological Society of America (GSA)
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