ISSN:
1432-1017
Keywords:
Nuclear magnetic resonance
;
OR3 operator
;
bacteriophage λ
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract Sequence-specific 1H NMR assignments are presented for a non-selfcomplementary 23-base-pair DNA duplex of molecular weight 15,000 daltons, containing the OR3 repressor binding site of bacteriophage λ as the central core. The NMR techniques used were mainly phase-sensitive two-dimensional NOE and 2Q spectroscopy, the latter to overcome overlap problems within the spectral region of the deoxyribose spin-systems. Direct sequential NOE connectivities are observed between adenine 2H and deoxyribose 1′ protons. We propose the use of these connectivities as a check of the assignments of C1′ and A2 protons, which have independently been derived via other assignment pathways.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00254064
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