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  • 1
    Electronic Resource
    Electronic Resource
    Changes in light sensitivity of Paulownia tomentosa and P. fortunei seeds
    Amsterdam : Elsevier
    Plant Science 39 (1985), S. 13-16 
    Details
    ISSN: 0168-9452
    Keywords: Light sensitivity ; Paulownia fortunei ; Paulownia tomentosa ; seed germination ; skotodormancy
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(85)90185-2
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of deuterium oxide on light-sensitive Paulownia tomentosa seed germination
    Amsterdam : Elsevier
    Plant Science 48 (1987), S. 37-42 
    Details
    ISSN: 0168-9452
    Keywords: Paulownia tomentosa ; deuterium oxide ; light sensitivity ; seed germination
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(87)90067-7
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  • 3
    Electronic Resource
    Electronic Resource
    Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 416-423 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218443
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  • 4
    Electronic Resource
    Electronic Resource
    Light and nitrate interaction in phytochrome-controlled germination ofPaulownia tomentosa seeds (1990)
    Springer
    Planta 181 (1990), S. 239-243 
    Details
    ISSN: 1432-2048
    Keywords: Light and germination ; Nitrate and seed germination ; Paulownia ; Phytochrome and seed germination ; Seed germination (light, nitrate)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pulsed light and nitrate exhibit an interactive effect on the germination ofPaulownia tomentosa Steud. seeds that require long periods of light irradiation. Two pulses of red light (R), separated by an adequately long dark interval, substitute for continuous prolonged irradiation. A far-red (FR) pulse given at the beginning of the dark interval inhibits germination, while it has no effect if given at the end. The requirement for certain ratios of the far-red-absorbing form of phytochrome/total phytochrome (Pfr/Ptot) differs when a FR+R-pulse is given as the first or second of two pulses (FR+R or R) separated by a dark interval. An equal decrease of the Pfr/Ptot ratio leads to a more pronounced decrease in germination when the pulse of the same FR+R ratio is given as the second pulse at the end of the dark interval. The length of dark interval between light pulses needed for maximal germination, differed in (i) seeds with a natural requirement for long periods of light irradiation from that in (ii) seeds with their long light requirement imposed by two weeks of imbibition in darkness or by (iii) imbibition in 40% heavy water. However, a single R pulse was sufficient to induce a high percentage of germination if the seeds were supplied with KNO3 (10 mM) from the onset of imbibition up to the onset of light. This effect decreased with a delayed time of application, and was prevented if FR preceded the KNO3 application.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02411545
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  • 5
    Electronic Resource
    Electronic Resource
    Gibberellic acid—Binding proteins from pea stems (1976)
    Springer
    Planta 131 (1976), S. 125-128 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The formation of complexes of gibberellic acid (GA3) and proteins under in vitro conditions was studied. It was shown that labelled GA3 binds to soluble cytoplasmic proteins, although a considerable amount of radioactivity remains in the pellet containing nuclei and cell debris. GA3-protein complexes are excluded from Sephadex G-10 column with the void volume. They sediment in linear sucrose density gradients as three distinct peaks, having higher S values than bovine serum albumin, used as a marker. Soluble GA3-protein complexes can be separated into four zones of radioactivity upon ion exchange chromatography on DEAE-Sephadex A-50 column, each of them eluting with a different KCl concentration. Agarose gel electrophoresis of GA3-protein complexes reveals two zones of radioactivity at the anodic part of the electrophoretogram. After extraction of the complex with ethanol, more than 90% of radioactivity is found in the ethanolic phase, which indicates that the binding is not covalent. GA4+7 and GA13 decrease the binding of GA3 to cytoplasmic proteins for 30%, suggesting that some common binding sites exist at the same binding proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389982
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  • 6
    Electronic Resource
    Electronic Resource
    Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor (1995)
    Springer
    Applied microbiology and biotechnology 43 (1995), S. 416-423 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  The production of anthraquinones by Frangula alnus Mill. plant cells was used as a model system to evaluate the performance of a liquid-liquid extractive product-recovery process. The shake flask experiments have shown higher production of anthraquinones in cell suspension and flask cultures of calcium-alginate-immobilized cells when silicone oil was incorporated into the medium, compared to a control without silicone oil. An external-loop air-lift bioreactor, developed and designed for the production and simultaneous extraction of extracellular plant cell products, was regarded as a four-phase system, with dispersed gas, non-aqueous solvent and calcium-alginate-immobilized plant cells in Murashige and Skoog medium. Continuous extraction of anthraquinones by silicone oil and n-hexadecane inside the bioreactor resulted in 10–30 times higher cell productivity, compared to that of immobilized cells in a flask. Based on the mixing pattern, immobilized biocatalyst extraparticle and intraparticle diffusional constraints and the kinetics of growth, substrate consumption and product formation, a mathematical model was developed to describe the time course of a batch plant cell culture. The model showed satisfactory agreement with four sets of shake flask experiments and three bioreactor production cycles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050428
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  • 7
    Electronic Resource
    Electronic Resource
    Interaction of gibberellins and fusicoccin in growth retardant- and far red light-inhibited germination of lettuce seeds (2000)
    Springer
    Plant growth regulation 32 (2000), S. 91-96 
    Details
    ISSN: 1573-5087
    Keywords: AC 94,377 ; far-red light ; fusicoccin ; germination ; gibberellins ; growth retardants ; lettuce seeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Germination of lettuce seeds cv. May Queen iscompletely prevented either with 10 µM tetcyclacisor with continuous FR illumination. GA3 and the N-substituted phtalimide, AC 94,377, werepartially effective in overcoming tetcyclacis-inducedinhibition but ineffective on photoinhibited seeds. FCcompletely reversed tetcyclacis inhibition and inducedca. 60% germination in continuous FR light. Aninteraction between FC and GA3 (as well asbetween FC and AC 94,377) was evident in stimulationof germination under both inhibitory conditions.Interaction was calculated as a ratio of thepercentage of seeds germinated under the simultaneousaction of stimulators compared to their additiveeffect. This was 2.54 for tetcyclacis- and 2.95 forphotoinhibited seeds. It is concluded that thistype of interaction is promotive synergism. Themagnitude of the interaction was highest if theapplication of FC was delayed after GA3application, and the optimal time lag was 6 h fortetcyclacis-inhibited, or 24 h for photoinhibited seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006464608469
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of nitric oxide – releasing compounds on phytochrome – controlled germination of Empress tree seeds (1998)
    Springer
    Plant growth regulation 26 (1998), S. 175-181 
    Details
    ISSN: 1573-5087
    Keywords: dismutation ; electron paramagnetic resonance ; phytochrome ; nitric oxide donors ; seed germination ; spin-trapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using different nitric oxide releasing compounds and appropriate controls we have obtained data strongly suggesting the involvement of nitric oxide in the phytochrome controlled germination of Paulownia tomentosa seeds. Direct detection of nitric oxide, under various experimental conditions, was performed by a spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy. The addition of methylene blue prevented light-induced and NO donors-potentiated germination of P. tomentosa seeds. This inhibition could be completely overcome by addition of gibberellin. The promotive effect of nitrite was pH dependent, maximally pronounced at the pH range where nitrite undergoes dismutation and liberates nitric oxide. Under these conditions, nitrite exerted its efficacy at the same concentrations at which nitric oxide releasing compounds such as sodium nitroprusside (SNP), S-nitroso acetylpenicillamine (SNAP), and 3-morpholinosydnonimine (SIN-1), were the most effective. Likewise, the potentiation of P. tomentosa seed germination could be achieved by chemical reduction of nitrite with Na2S2O4 during which liberation of nitric oxide could be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006131215297
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  • 9
    Electronic Resource
    Electronic Resource
    Lenticel hypertrophy in shoot cultures of Ceratonia siliqua L. (1992)
    Springer
    Plant cell, tissue and organ culture 31 (1992), S. 111-114 
    Details
    ISSN: 1573-5044
    Keywords: Carob ; Ceratonia siliqua L. ; in vitro ; lenticels ; lenticel hypertrophy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Numerous white surface proliferations appeared in cultures of Ceratonia siliqua L. grown three to four weeks on medium containing 0.5 mg l-1 BA and 0.1 mg l-1 IBA. It was histologically confirmed that these proliferations were hypertrophied lenticels. Proliferations appeared first at the basal shoot internode and gradually spread acropetally, covering eventually the whole shoot except the uppermost internodes. Increase of BA concentration in the medium increased both the number of hypertrophied lenticels per shoot and the shoot multiplication index.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037694
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  • 10
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    Production of anthraquinones by immobilized Frangula alnus Mill. plant cells in a four-phase air-lift bioreactor (1995)
    Springer
    Details
    Publication Date: 1995-07-01
    Print ISSN: 0175-7598
    Electronic ISSN: 1432-0614
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer
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