ISSN:
1608-3245
Keywords:
oligodeoxynicleotide
;
phosphodiester bond
;
terminal phosphate activation
;
conjugate
;
functional groups
;
chemical modification
;
synthesis
;
probe
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The article describes the optimized methods for covalent attachment of unprotected oligonucleotides to functionally important ligands through their terminal phosphate or thiophosphate, including covalent attachment of one oligonucleotide to another. A comparative description of methods is presented for selective introduction of the phosphate, thiophosphate, amino, sulfhydryl, aldehyde, carboxylic, and other groups into the terminal nucleotide using chemical and enzymatic reactions both in aqueous and organic media. Depending on their chemical nature, these groups can then interact with electrophilic or nucleophilic ligands carrying aliphatic or aromatic amino groups, hydrazido, sulfhydryl, disulfide, carboxylic, hydroxyl, aldehyde, bromo- or iodoalkyl, isothiocyanate, and other functions. The available methods allow one to vary the size of the linker between the oligonucleotide and ligand, its hydrophobicity and stability in acidic or alkaline media. The use of the disulfide bond permits cleavage of the oligonucleotide–ligand linkage in mild conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026667523884
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