ISSN:
0006-3525
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
The buoyant densities of natural and synthetic DNA's can be accurately interrelated if second-neighbor influences are taken into account. We derive the following expressions, based partly on the buoyant densities of six synthetic DNA's, for the buoyant densities ρ (g/cm3) of DNA's having random sequences. In CsCl, \documentclass{article}\pagestyle{empty}\begin{document}$$ \rho = 1.659 + 0.099H_{\rm G} - 0.065\,H_{\rm G} ^2 + 0.080\,H_{\rm G} ^3 $$\end{document} and in Cs2SO4, \documentclass{article}\pagestyle{empty}\begin{document}$$ \rho = 1.417 + 0.011\,H_{\rm G} - 0.004\,H_{\rm G} ^2 + 0.035\,H_{\rm G} ^3 $$\end{document}. In these equations, HG is the mole fraction of G : C base pairs in the DNA and the buoyant densities are calculated relative to densities for E. coli DNA of 1.703 and 1.426 (g/cm3) in CsCl and Cs2SO4, respectively.
Additional Material:
1 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bip.1974.360131108
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