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  • 1
    Electronic Resource
    Electronic Resource
    The use of rootless mutants for the screening of spontaneous androgenetic and gynogenetic haploids in Nicotiana tabacum: evidence for the direct transfer of cytoplasm (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 13-15 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana tabacum ; Cytoplasm transfer ; in situ androgenesis ; Rootless mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In crosses between a homozygous rootless mutant line of Nicotiana tabacum used as female and other Nicotiana tabacum lines, androgenetic haploids can be directly selected by their ability to form plantlets with a normal rooting system, whereas hybrid plants are killed few weeks after sowing. These androgenetic plants have the nucleus of the male parent into the cytoplasm of the female parent. In crosses where the homozygous rootless mutant line is used as a pollen donor, gynogenetic haploids can also be directly selected. Haploids can therefore be derived from male sterile plants using this approach. A generalization of this system for direct cytoplasm transfer and for the screening of spontaneous haploids in dicotyledons is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249135
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  • 2
    Electronic Resource
    Electronic Resource
    Direct selection of cybrids by streptomycin and valine resistance in tobacco (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 11-14 
    Details
    ISSN: 1432-2242
    Keywords: Cybrid ; Streptomycin resistance ; Valine resistance ; Tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Direct selection of cybrids by simultaneous selection for “donor” chloroplasts and for the “recipient” nuclei is described. Mesophyll protoplasts of two tobacco (Nicotiana tabacum) mutants, SR1 (streptomycin resistant) and Valr-2 (valine resistant), were fused by polyethylene glycol treatment. Streptomycin resistance in the SR1 mutant is a maternally inherited chloroplast trait while valine resistance is a Mendelian (nuclear) digenic recessive character. The fused protoplast population was cultured and colonies were selected for resistance to valine (1 mM) and streptomycin (343 μM). The efficiency of selection has been confirmed in three clones by demonstrating seed transmission of both streptomycin and valine resistances. In one subclone both streptomycin resistant and sensitive plants were obtained indicating that the streptomycin sensitive chloroplasts had not been totally eliminated by growth on the selective medium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261447
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  • 3
    Electronic Resource
    Electronic Resource
    Cellular genetic study of a somatic instability in a tobacco mutant: in vitro isolation of valine-resistant spontaneous mutants (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 482-488 
    Details
    ISSN: 1432-2242
    Keywords: Somatic instability ; Valine resistance ; Nicotiana tabacum ; Protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A chlorophyll-deficient mutant line of tobacco (Nicotiana tabacum), named tl, displays spontaneously on leaves green, white, and twinned green/white somatic variations at high frequencies (10−3 to 10−2). The frequency of cell events leading to the somatic variations has been shown to be closely dependent on the stage of differentiation of cells during plant development. The activity of transposable elements is suspected in the tl genotype, and a study of its mutagenic ability was performed by selecting in vitro new mutant cellular types. The cellular marker chosen was the resistance to toxic doses of valine conferred by a permeation deficiency. A reproducible method allowing efficient selection of valine-resistant mutant clones from haploid tobacco mesophyll protoplast-derived cells was used. In 10 out of 12 experiments, the frequency of spontaneous valine-resistant clones obtained with the wild-type control was null for cell populations tested to the 106. On the other hand, spontaneous valine-resistant clones were repeatedly isolated at variable and sometimes high frequencies (greater than 10−3) from cell populations of the tl type. Valine resistance of plants regenerated from these clones was transmitted to the progeny as a single monogenic mutation. These results indicate an increased mutagenic ability of the tl genotype, as compared to the wild-type line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00274267
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and characterization of valine-resistant mutants of Nicotiana plumbaginifolia (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 272-277 
    Details
    ISSN: 1432-2242
    Keywords: Valine resistance ; Nicotiana plumbaginifolia ; Protoplast ; Amino acid uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Haploid mesophyll protoplasts of Nicotiana plumbaginifolia were mutagenized by UV-irradiation. Protoplast-derived colonies were then selected for valine resistance on a medium containing 5 or 10 mM valine. From the resistant calli, plants were regenerated. Resistance was inherited as a recessive Mendelian character in seven clones. Mutations conferring valine resistance were shown to be allelic. Protoplast-derived cells of L-valine-resistant plants were also resistant to L-threonine. Resistance to valine was based on a reduced valine uptake rate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303963
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  • 5
    Electronic Resource
    Electronic Resource
    Transfer of cytoplasmic male sterility by spontaneous androgenesis in tobacco (Nicotiana tabacum L.) (1992)
    Springer
    Euphytica 66 (1992), S. 45-53 
    Details
    ISSN: 1573-5060
    Keywords: cytoplasmic male sterility ; Nicotiana tabacum ; spontaneous androgenesis ; Rac- mutant ; chromosome doubling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Five cytoplasmic male sterile lines of Nicotiana tabacum homozygous for a dominant rootless mutation [Rac-] were used as female parents in crosses with three male fertile varieties. Androgenetic haploids were selected by their ability to form plantlets with a normal root system, whereas hybrid plantlets failed to grow. High frequencies of androgenetic haploid plants were obtained with N. debneyi cytoplasm, and with one paternal genotype. Chromosome doubling was performed using three different methods (pollination, in vitro culture, acenaphthen) and cytoplasmic male sterile doubled haploid plants have been obtained. The results demonstrate the efficiency of this method for a rapid cytoplasm transfer in Nicotiana tabacum; the integrity of the cytoplasm is conserved. For the first time, we possess a counter selectable marker universally usable for Nicotiana tabacum and closely related species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023507
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