ISSN:
1432-0983
Keywords:
Key words Trichoderma
;
Proteasome
;
Unfolded protein stress response
;
ER quality control
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have cloned a gene, prs12, from the filamentous fungus Trichoderma reesei which encodes a fungal homologue of the mouse and Drosophila regulatory subunit 12 of the 26S proteasome (mov34). Sequencing of both a genomic and a cDNA-clone predicts a 342-aa protein with high overall identity (56–68 %) to the homologous counterparts from human, mammals, Drosophila and Saccharomyces cerevisiae. The predicted protein contains several consensus sequences for phosphorylation, three of which are conserved in all published Prs12p homologues. Its C-terminus is rich in alternating K and E/D, and resembles a potential KEKE-motif. Prs12 exhibits a basal level of transcription during normal growth, but its expression is significantly increased over 60–120 min under conditions of stress evoked by the addition of cadmium ions and hygromycin B. It is also stimulated by the addition of tunicamycin and 2-mercaptoethanol, suggesting its regulation by the presence of unfolded proteins in the endoplasmic reticulum and by hygromycin B. Consistent with this behaviour, motifs in the prs12 5′-upstream sequences show sequence homology with the consensus sequences for general stress response, and for an ER traffic-response element.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050338
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