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  • 1
    Electronic Resource
    Electronic Resource
    Alkaloid production in Catharanthus roseus cell cultures (1982)
    Springer
    Plant cell reports 1 (1982), S. 139-142 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus derived from hypocotyls of periwinkle, Catharanthus roseus, responded to culture on nutrient media supplementedwith IAA, BA, and zeatin with shoot formation at low frequencies. However, shoot regenerating callus could be very successfully propagated and subcultured. Alkaloid profiles of callus derived from the original explants (hypocotyls) as well as callus derived from regenerated shoots were almost identical. Subcultures of old callus (initiated in 1978) failed completely to grow shoots. In programs for long-term preservation of alkaloid producing cell lines by regeneration and storage of shoots, selection for ability to form shoots would have to precede selection for alkaloid production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269182
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  • 2
    Electronic Resource
    Electronic Resource
    Cryopreservation of periwinkle, Catharanthus roseus cells cultured in vitro (1982)
    Springer
    Plant cell reports 1 (1982), S. 135-138 
    Details
    ISSN: 1432-203X
    Keywords: Catharanthus roseus ; periwinkle ; cell cultures ; DMSO ; cryopreservation ; cryogenic storage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure for prolonged cryogenic storage of periwinkle cell cultures is described. Cells derived from periwinkle, Catharanthus roseus (L.) G. Don, and subcultured as suspension in 1-B5C nutrient medium have been frozen, stored in liquid nitrogen (−196°C) for 11 weeks, thawed and recultured. Maximal survival was achieved when 3–4 day-old cells precultured for 24 h in nutrient medium with 5% DMSO were frozen at slow cooling rates of 0.5 or 1°C/min prior to storage in liquid nitrogen. The only loss in viability of cells occurred subsequent to treatment with DMSO. Abbreviations: DMSO, dimethylsulfoxide; 2,4-D, 2,4-dichlorophenoxyacetic acid; TTC, triphenyltetrazolium chloride.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269181
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    Paper (German National Licenses)
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