ISSN:
0006-3592
Keywords:
microfiltration
;
protein filtration
;
membrane fouling
;
standard blocking filtration law
;
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
An experimental study of permeation of dilute BSA solutions (filtration) at microfiltration membranes has been carried out. Most measurements were made with capillary pore aluminum oxide membranes, with some comparative measurements with tortuous and capillary pore polymeric membranes. In all cases, a continuous and substantial decrease in the rate of permeation with time was observed. This decrease in permeation with time was observed. This decrease in permeation rate was due neither to concentration polarization nor to protein adsorption alone. However, it could be quantified using the standard blocking filtration law, which describes a decrease in pore volume due to deposition of protein on the walls of the pore. The maximum calculated thickness of the deposited layers was 55nm on the walls of 200-nm diameter pores. This phenomenon is quite different to adsorption of protein at such surfaces, this latter giving only sub-monolayer or monolayer protein coverage under the conditions studied.
Additional Material:
14 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/bit.260380703
Permalink