Publication Date:
2009-01-01
Description:
AflatoxinB1(AFB1) is a carcinogenic metabolite produced by certainAspergillusspecies on agricultural commodities. AFB1biosynthesis is affected by jasmonic acid and also by its methylester (MeJA), a plant growth regulator derived from linoleic acid. This study reports the effect of MeJA on the growth ofA. parasiticusand AFB1output in yeast extract sucrose (YES) medium when added at three different concentrations; namely,10−2 M,10−4 M, and10−6 M. AFB1determination was performed by immunoaffinity and HPLC. MeJA at10−4and10−6M concentrations had no significant effect on mycelial growth but did affect AFB1production after the 7th day of incubation; on the 12th day, AFB1production was increased by 212.7% and 141.6% compared to the control samples (addition of10−6 M and10−4 M MeJA, resp.). Treatment ofA. parasiticuscultures with10−2 M MeJA inhibited mycelial growth and AFB1production as well. These results suggest that the effect of MeJA on AFB1biosynthesis byA. parasiticusdepends on the MeJA concentration used.
Print ISSN:
1687-918X
Electronic ISSN:
1687-9198
Topics:
Biology
,
Medicine
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