ISSN:
1550-7408
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
. Targeted gene disruption was used to investigate the function of MYO1, an unconventional myosin gene in Tetrahymena thermophila. Phenotypic analysis of a transformed strain that lacked a functional MYO1 gene was conducted at both 20°C and 35°C. At either temperature the ΔMYO1 strain had a smaller cytoplasm/nucleus ratio than wild type. At 20°C, ΔMYO1 populations had a longer doubling time than wild type, lower saturation density, and a reduced rate of food vacuole formation. However, at 35°C, these characteristics were comparable to wild type. Although micronuclear division and cytokinesis appeared normal in ΔMYO1 cells, failure of the macro-nucleus to elongate properly resulted in unequal segregation of macronuclear DNA in cells maintained at either 20°C or 35°C.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1550-7408.2000.tb00090.x
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