ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Sarcoplasmic proteins from beef skeletal muscle were fractionated by chromatography on ion-exchange cellulose (cellulose phosphate and diethyl-aminoethyl cellulose) at pH 5 and 9.3. From effluent diagrams of sarcoplasma from muscle immediately after slaughter, at least 14 fractions were recognized, and some of them were identified with known proteins by assays for enzymic activities. The numbers and levels of eluted peaks in the effluent diagram were found to decrease with storage (aging) of muscle and freeze-drying of sarcoplasma; these decreases are perhaps due to denaturation. Proteins in solutions appear to be more completely separated by chromatography when different combinations of ion-exchange cellulose are used.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1964.tb01737.x
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