ISSN:
0739-4462
Keywords:
larval midgut enzymes
;
adult midgut enzymes
;
housefly midgut lysozyme
;
digestion
;
ontogenesis
;
Chemistry
;
Food Science, Agricultural, Medicinal and Pharmaceutical Chemistry
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
Notes:
Based on polyacrylamide gel electrophoresis, density-gradient ultracentrifugation and thermal inactivation, there is only one major molecular species of each of the following larval enzymes (soluble in water or solubilized in Triton X-100): membrane-bound aminopeptidase (pH optimum 8.5; Km 0.21 mM L-leucine p-nitroanilide; Mr 322,000), amylase (pH optimum 6.5; Km 0.14% starch; Mr 66,000), lysozyme (pH optimum 3.5; Km 0.3 mg/ml; Mr 24,000); and membrane-bound trehalase (pH optimum 5.0; Km 1.09 mM trehalose; Mr 94,000). Except for lysozyme, the properties of adult digestive enzymes are different from those described for larval enzymes. Larval aminopeptidase and trehalase were purified by electrophoresis and larval lysozyme (contaminated with amylase) by density-gradient ultracentrifugation, and were used to raise antibodies in a rabbit.Antibodies raised against larval aminopeptidase, trehalase, and amylase did not recognize the imaginal enzymes, whereas those against larval lysozyme recognize imaginal lysozyme. The data suggest that the genes coding for digestive enzymes (except for lysozyme) are different in larvae and imagoes.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/arch.940090404
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