ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract The binding of 125I-labelled fibronectin and its 125I-labelled amino-terminal (29 kDa) domain to smooth Salmonella strains and rough variants was studied. 11 Of the 14 strains bound fibronectin, in the order of 20–70% of total radioactivity added. The kinetics of binding to Salmonella strain 27655R for the intact fibronectin and the 29 kDa fragment was very rapid, binding being completed within 20 min. Binding was specific, since the unlabelled fibronectin and the 29 kDa fragment could compete efficiently for binding of 125I-labelled fibronectin and fragment, whereas other glycoproteins, such as ovalbumin, fetuin and orosomucoid, showed very little or no inhibition. Since the unlabelled 29 kDa fragment could efficiently inhibit 125I-labelled fibronectin binding, it seems likely that the site of interaction is the amino-terminal domain of fibronectin.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1985.tb00752.x
Permalink