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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 18 (1983), S. 148-152 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A new alcohol tolerant yeast which had the ability to ferment glucose, galactose, maltose, trehalose, melizitose, α-methyl-d-glucoside, melibiose and raffinose (complete) was isolated from fermented rice wine. This new isolate which was morphologically, culturally and physiologically similar to type species Saccharomyces uvarum Beijerinck was considered as a variety of the species, hence, designated as Saccharomyces uvarum inulyticus var. nov., because of its extraordinary ability to ferment inulin into ethanol and hyper-tolerance to high concentration of ethanol at a wide range of temperature. The new variant produced 21.6%, 20%, 18.6%, and 9.8% v/v ethanol at 15, 20, 30, and 40° C, respectively, when fermentation was carried out by the stepwise addition of sucrose to the synthetic medium. The addition of fungal mycelia into the synthetic medium increased the fermentation rate and ethanol concentration up to 22.4%, 21.8%, 20.1%, and 11.6% v/v at 15, 20, 30, and 40° C, respectively. The new variant was found to grow well at 40° C and did not require any vitamin or growth factor, such as biotin, calcium pantothenate, inositol, niacin, p-aminobenzoic acid, pyridoxine hydrochloride, riboflavin and thiamine hydrochloride for normal growth.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 25 (1983), S. 1973-1980 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A simple method of preparing a chitin-immobilized α-amylase and glucoamylase from the protease- and glycosidase-less Mutant HF-15 of Aspergillus awamori var. kawachi was developed and used for the production of high-glucose syrup. The glucoamylase was tightly bound onto chitin without the aid of a crosslinking agent because the enzyme contained a specific binding site for chitin. Continuous production of high glucose concentrate from a highly concentrated α-amylasetreated gelatinized starch substrate (about 45% total solids) was undertaken successfully with the use of a column-packed chitin-immobilized amylase. The activity of the column was stable for more than 20 days of continuous operation and the product was found to be only glucose with an average dextrose equivalent value of more than 97%. The final product showed no isomaltose or panose contamination, indicating that the immobilized amylase had no transglucosidation activity. The immobilized amylase was most active in the conversion of gelatinized starch to glucose at 55°C and pH 2.5 to 5.0. Drying the chitin-immobilized amylase resulted in the decrease of activity and shortening of storage life, whereas a storage life of up to 80 days was attained without affecting its original activity when kept under moist condition at 4°C.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Publication Date: 1983-01-01
    Print ISSN: 0171-1741
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Published by Springer
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