ISSN:
1617-4623
Keywords:
Interferon-γ
;
Mutagenesis
;
Signal transduction
;
Indoleamine 2,3-dioxygenase
;
Gene expression
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Our previous observations indicated that mutants partially resistant to IFN-y cytotoxicity were defective in the induction of indoleamine 2,3-dioxygenase, (IDO). Two mutants highly resistant to IFN-γ were isolated following a second round of mutagenesis. The resistance to IFN-γ was inversely correlated with the inducibility of IDO in these mutants. Moreover, several other IFN-γ responsive genes, including those encoding 2-5A synthetase, GTP cyclohydrolase and HLA-DRα, were also differentially altered in their expression upon INF-γ treatment. IFN-y receptor gene expression was not changed nor was the binding of the receptor to IFN-γ. Southern blot analysis failed to reveal any significant abnormality in the IDO gene structure in these mutants. We therefore suggest that these mutants are defective in the IFN-γ signaling pathway and will be useful in further analysis of the biochemical mechanism of IFN-γ activated gene expression in target cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00290655
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