ISSN:
0025-116X
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
The helix-coil transition in Phage T2 DNA in the presence of 6,4 · 10-3 mol/l Mn2+ is studied using light scattering and UV spectroscopy. The transition range is about 0,5°C. Near the temperature of the end of melting Tf the molecular weight Mw and the radius of gyration Rz of the complex are observed to decrease to about one half. At a temperature 0,1-0,25°C higher than Tf, Mw and Rz pass through a minimum, which implies that aggregation is preceded by unwinding of DNA strands. Thus, rise in temperature rather than Mn2+ -induced aggregation causes DNA + Mn2+ melting.
Additional Material:
3 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/macp.1987.021881006
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