Publication Date:
2002-10-05
Description:
The emergence of insecticide resistance in the mosquito poses a serious threat to the efficacy of many malaria control programs. We have searched the Anopheles gambiae genome for members of the three major enzyme families- the carboxylesterases, glutathione transferases, and cytochrome P450s-that are primarily responsible for metabolic resistance to insecticides. A comparative genomic analysis with Drosophila melanogaster reveals that a considerable expansion of these supergene families has occurred in the mosquito. Low gene orthology and little chromosomal synteny paradoxically contrast the easily identified orthologous groups of genes presumably seeded by common ancestors. In A. gambiae, the independent expansion of paralogous genes is mainly a consequence of the formation of clusters among locally duplicated genes. These expansions may reflect the functional diversification of supergene families consistent with major differences in the life history and ecology of these organisms. These data provide a basis for identifying the resistance-associated enzymes within these families. This will enable the resistance status of mosquitoes, flies, and possibly other holometabolous insects to be monitored. The analyses also provide the means for identifying previously unknown molecules involved in fundamental biological processes such as development.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Ranson, Hilary -- Claudianos, Charles -- Ortelli, Federica -- Abgrall, Christelle -- Hemingway, Janet -- Sharakhova, Maria V -- Unger, Maria F -- Collins, Frank H -- Feyereisen, Rene -- U01 AI48846/AI/NIAID NIH HHS/ -- U01 AI50687/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2002 Oct 4;298(5591):179-81.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12364796" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Anopheles/enzymology/*genetics
;
Carboxylic Ester Hydrolases/chemistry/classification/*genetics/metabolism
;
Computational Biology
;
Conserved Sequence
;
Cytochrome P-450 Enzyme System/chemistry/classification/*genetics/metabolism
;
Drosophila melanogaster/enzymology/genetics
;
*Evolution, Molecular
;
Genes, Duplicate
;
Genes, Insect
;
Genome
;
Genomics
;
Glutathione Transferase/chemistry/classification/*genetics/metabolism
;
Insecticide Resistance/*genetics
;
Multigene Family
;
Mutation
;
Phylogeny
;
Physical Chromosome Mapping
;
Sequence Analysis, DNA
;
Terminology as Topic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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