ISSN:
1617-4623
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
Notizen:
Summary A high proportion of intracellular λ DNA molecules are found to have D-loops, when isolated under four different conditions: (1) λOts after 7 min at 31°C in the presence of chloramphenicol; (2) λOts after 7 min at 31° C without chlormaphenicol; (3) λOst after 30 min at 42° C; and (4) λcIIcIII after 50 min at 37° C. The great majority of these D-loops contain RNA and are produced by E. coli RNA polymerase. In the presence of chloramphenicol, D-loops are mostly limited to the immediate early regions of the major leftward and rightward operons. At early times, with no chloramphenicol present, D-loops map primarily within the delayed early regions of the two major operons. At late times, D-loops are found mostly within the major late operon of the bacteriophage DNA. This physical evidence corroborates evidence of the temporal transition in λ transcription obtained by other means. Chloramphenicol is shown to block the transition from immediate early to delayed early transcription.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00337964
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