ISSN:
1399-3054
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Lipids and pigments of the chlorophyll b-deficient mutant pg-113 and the parent strain (ps) of Chlamydomonas were analysed and compared. Monogalactosyldiglyceride, digalactosyldiglyceride, diacylglyceryl(N, N, N-trimethyl)homoserine, sulfoquinovosyldiglyceride, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol were found as major lipid components. While the lipid patterns were qualitatively and quantitatively almost the same in the two strains, the C16/C18 fatty acid ratios were different, 0.85 in the mutant and 1.11 in the parent strain. Furthermore, the relative amounts of C16- and C18-monoene fatty acids were slightly enhanced and the C18-trienes slightly reduced in the mutant. In the parent strain, chlorophylls a and b, α- and β-carotene, lutein, violaxanthin, neoxanthin and loroxanthin were detected by HPLC. In the mutant, similar pigments were found, except that only traces of chlorophyll b and a reduced amount of neoxanthin were present. Since no chlorophyll-protein complex CP II could be detected in the mutant by electrophoresis, the possible interrelationships between pigment deficiency and alteration of chlorophyllprotein complexes are discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1399-3054.1986.tb05584.x
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