ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract Heterologous DNA hybridization using a ribulose- 1,5-biphosphate carboxylase/oxygenase (RuBisCOase) large subunit gene (rbcL) probe from Anacystis nidulans revealed the presence of two rbcL in Nitrobacter hamburgensis. One gene is located on a plasmid, the other on the chromosome. The genes appear to be very similar since both hybridized strongly to the A. nidulans probe. However, restriction endonuclease digestions revealed differences.Two different RuBisCOase enzymes were isolated from N. hamburgensis. The Mr of the native enzymes were 520 000 and 480 000. Sodium dodecyl sulfate-polycrylamide gel electrophoresis (SDS-PAGE) revealed the presence of both LSU and small subunits (SSU) for both enzymes. The Mr were 53 000 and 16 000, and 49 000 and 13 500, respectively. A hexadecameric structure is suggested for both enzymes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1988.tb02728.x
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